| High on a Mountain | Ola Belle Reed | 5:30 | |
| All Night Long - Remastered | Miles Pratcher, Bob Pratcher, Alan Lomax | 3:04 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| Bad Luck Everywhere You Go | C.W. Stoneking | 4:22 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Pretty Polly Oliver - Remastered | Ollie Gilbert, Alan Lomax | 2:33 | |
| Buzzard Lope | Bessie Jones, Georgia Sea Island Singers, Alan Lomax | 1:59 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Mother's Last Word To Her Son | Washington Phillips | 2:52 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Caberfeidh | John Burgess, Alan Lomax | 2:36 | |
| Handyman Blues | C.W. Stoneking | 3:24 | |
| Diddie Wa Diddie | Blind Blake | 3:00 | |
| Ramblin' On My Mind | Robert Johnson | 2:53 | |
| Rosie | Unidentified Mississippi State Penitentiary (Parchman Farm) prisoners, Alan Lomax | 2:55 | |
| There's a New World Coming | Bernice Johnson Reagon | 3:37 | |
| Rambling Gambler | Alan Lomax | 3:10 | |
| Every Time I Feel the Spirit | The Zion Travelers | 2:44 | |
| Dangerous Blues - Remastered | Floyd Batts, Alan Lomax | 1:07 | |
| Jelili (Ielili) | Akbar Jaffarov, Museyib Abbasov, Alan Lomax | 3:57 | |
| Empty Bed Blues, Pt. 1 | Bessie Smith | 3:00 | |
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| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Grinnin' In Your Face | Son House | 1:56 | |
| Goodbye Honey, You Call That Gone | Lucius Smith, Alan Lomax | 2:13 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Good Luck Charm | C.W. Stoneking | 2:45 | |
| Wave the Ocean - Remastered | Charlie Everidge, Neal Morris, Alan Lomax | 1:37 | |
| Cross Road Blues - Take 1 | Robert Johnson | 2:37 | |
| Ocean Of Tears | Big Maybelle | 2:29 | |
| The Devil's Dream | Sid Hemphill, Alan Lomax | 3:09 | |
| Jumping Judy | Allen Prothero, Alan Lomax | 2:55 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Sally Anne | Alan Lomax, Norman Edmonds & the Old-Timers | 2:26 | |
| Shoo Turkey | Bessie Jones, Alan Lomax | 1:04 | |
| I'm Tired - Remastered | Bright Light Quartet, Alan Lomax | 2:51 | |
| Saviour, Pass Me Not | Swan Silvertones | 5:07 | |
| Fixin' to Die Blues | Bukka White | 2:48 | |
| John Henry | Henry Thomas | 2:43 | |
| Black Woman | Alan Lomax | 2:57 | |
| Come And Go With Me To That Land | Bernice Johnson Reagon | 4:40 | |
| Get Away Jordan | Dorothy Love Coates, The Original Gospel Harmonettes | 2:45 | |
| Peace In The Valley | Sam Cooke, The Soul Stirrers | 2:45 | |
| Haunted House | Lonnie Johnson, Elmer Snowden | 5:00 | |
| Country Blues (No. 1) - Plantation Recording | Muddy Waters | 3:33 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Mother Earth | Memphis Slim | 2:39 | |
