| Guitar Highway | Brownie McGhee, Sonny Terry | 2:35 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Worried Life Blues | Big Maceo | 2:54 | |
| Dig Myself a Hole | Arthur "Big Boy" Crudup | 2:23 | |
| Junker's Blues | Champion Jack Dupree | 3:12 | |
| I Wish You Would | Billy Boy Arnold | 3:39 | |
| Have Mercy | Big Walter Horton | 3:45 | |
| Sweet Home Chicago | Robert Johnson | 2:59 | |
| Back Home Blues | Big Joe Williams | 4:22 | |
| Lonesome Bedroom Blues | William Clarke | 5:45 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Move to Kansas City | Brownie McGhee | 3:06 | |
| Hen Cackle | Woody Guthrie, Cisco Houston, Sonny Terry | 2:54 | |
| When the Levee Breaks | Memphis Minnie | 3:11 | |
| Little Boy Blues | Robert Lockwood, Jr. | 3:06 | |
| When Death Come Creeping in Your Room (Run, Sinner, Run) - Take 1; Remastered Version | Mance Lipscomb | 2:57 | |
| The Blues Had a Baby | Brownie McGhee | 2:56 | |
| What You Doing to Me (feat. Victor Wainwright) | Brandon Santini, Victor Wainwright | 5:18 | |
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| Richmond Blues | Cephas & Wiggins, Phil Wiggins, John Cephas | 3:59 | |
| Blues for Nothing | Mike Bloomfield, Al Kooper | 4:15 | |
| Harmonica Blues | Sonny Terry | 4:42 | |
| Temptation Blues | Big Walter Horton | 3:43 | |
| Mojo Hand | Lightnin' Hopkins | 2:59 | |
| Take A Walk With Me (Take2) | Robert Lockwood, Jr., JR. | 3:00 | |
| The Man Next Door | Koko Taylor | 5:15 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| Another Man Done Gone | Sugar Blue | 2:18 | |
| Hastings Street | Blind Blake | 3:10 | |
| Ain't Times Hard | Nick Moss & The Flip Tops, Barrelhouse Chuck, Kate Moss | 4:01 | |
| Downtown Blues | Frank Stokes | 3:17 | |
| Hey Now Baby | Professor Longhair | 5:44 | |
| Two Bugs and a Roach | Earl Hooker | 4:19 | |
| Blue and Worried Man | Blind Boy Fuller, Sonny Terry | 2:43 | |
| Catfish Blues | Skip James | 4:37 | |
| Dark Road | Floyd Jones | 3:37 | |
| What A Dream | Slim Harpo | 3:06 | |
| Woman I Ain't Gonna Drink No More Whiskey | Sunnyland Slim | 3:11 | |
| That's All Right - 1950 Single Version | Jimmy Rogers | 2:50 | |
| Frosty - Single Version | Albert Collins | 3:05 | |
| The Devil Is A Busy Man | Sunnyland Slim | 3:56 | |
| Take It Easy | Pinetop Perkins | 3:43 | |
| Corrinna, Corrinna | Mississippi John Hurt | 1:51 | |
| Sloppy Drunk - Single Version | Jimmy Rogers | 3:02 | |
| Left My Happy Home | Billy Boy Arnold | 2:34 | |
| Ice Cream Man | John Brim | 2:44 | |
| Homesick's Blues | Homesick James | 3:08 | |
