| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| Tomorrow Gon Be Too Late | C.W. Stoneking | 3:05 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Girl of Constant Sorrow | Barbara Dane | 4:06 | |
| Brunswick Stew | Kacy & Clayton | 3:10 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Ramblin' Boy | Tom Paxton | 3:58 | |
| Rudee Down in New Orleans | Blaze Foley | 2:07 | |
| La Le Too Dum | Barbara Dane | 3:18 | |
| In Younger Days | GeeGee's Rhythm Band | 2:45 | |
| East Virginia | Nora Brown | 4:33 | |
| Oval Room | Blaze Foley | 4:04 | |
| Jane, Jane | Tia Blake | 3:37 | |
| You'll Get Yours Aplenty | Blaze Foley | 2:09 | |
| My Baby Is Coming Back To Me | Abner Jay | 2:17 | |
| Dirty Old Town | Ewan MacColl | 1:03 | |
| How Long | C.W. Stoneking | 3:13 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Fish Ain't Bitin' | Corey Harris | 3:28 | |
| It's Alright | Precious Bryant | 1:47 | |
| I Never Will Marry | The Kossoy Sisters | 2:48 | |
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| Who's Gonna' Shoe Your Pretty Little Foot | Barbara Dane | 3:43 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Big Cheeseburgers & Good French Fries | Blaze Foley | 2:14 | |
| Frosty Morn | The Doc Watson Family | 1:41 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Down in a Willow Garden | The Kossoy Sisters | 2:34 | |
| Georgia Buck | Precious Bryant | 2:38 | |
| God Bless the Grass | Malvina Reynolds | 2:04 | |
| Fine Artiste Blues | R. Crumb And His Cheap Suit Serenaders | 3:06 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Goin' Back South | C.W. Stoneking | 3:30 | |
| Knife Guitar | Roscoe Holcomb | 1:17 | |
| When I Get Back Home | Michael Hurley | 6:05 | |
| Bold Ruler's Joys | Nathan Salsburg | 2:17 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Don't Lose Your Cool | Ted Hawkins | 2:46 | |
| Bye Bye Blackbird | Earl Hines, Kenny Whitson, Barbara Dane, Benny Carter, Herbie Harper, John Halliburton, Plas Johnson, Leroy Vinnegar, Shelly Manne, Wellman Braud, Billy Strange, Jesse Sailes, Earl Palmer, Rocco Wilson, Ray Johnson | 2:27 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Celebrations For A Grey Day | Mimi And Richard Farina | 3:48 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Sweet Lucy | Michael Hurley, Unholy Modal Rounders, Jeffrey Frederick & The Clamtones | 4:06 | |
| One-Dime Blues | Etta Baker | 2:35 | |
| Samson And Delilah | Rev. Gary Davis | 4:03 | |
| Moon Over Montana - Mono | Jimmy Wakely | 2:57 | |
| Tell Me You Love Me | Jessie Mae Hemphill | 2:46 | |
| House Un-American Blues Activity Dream | Mimi And Richard Farina | 3:12 | |
| Mecca Flat Blues | Earl Hines, Kenny Whitson, Barbara Dane, Benny Carter, Herbie Harper, John Halliburton, Plas Johnson, Leroy Vinnegar, Shelly Manne, Wellman Braud, Billy Strange, Jesse Sailes, Earl Palmer, Rocco Wilson, Ray Johnson | 2:59 | |
| Fenario | The Journeymen | 2:49 | |