| La danse de Mardi Gras | The Balfa Brothers | 2:22 | |
| Let the Sun Shine Down on Me - Remastered | Jean Ritchie | 2:22 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Bukka Jitterburg Swing | Bukka White | 2:35 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| Beer Drinking Woman | Memphis Slim and Willie Dixon | 2:32 | |
| God's Word | J.B. Lenoir | 3:41 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Sally, Where'd You Get Your Liquor From | Rev. Gary Davis | 1:56 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| God Don't Ever Change | Corey Harris | 2:10 | |
| The House Carpenter - 2020 Remaster | Clarence Ashley | 3:18 | |
| Diamond Joe | Bessie Jones | 1:19 | |
| Mother's Last Word To Her Son | Washington Phillips | 2:52 | |
| Prettiest Train | Alan Lomax | 3:43 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:05 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3:18 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
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| Stewball | Unidentified Mississippi State Penitentiary (Oakley) prisoners, Alan Lomax | 4:10 | |
| One Dime Blues | Etta Baker | 3:43 | |
| Aunt Caroline Dyer Blues | Memphis Jug Band | 3:06 | |
| Got to Lie Down | Bessie Jones | 2:27 | |
| Hold My Body Down | Robert Johnson | 2:41 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Adam in the Garden | Bessie Jones, Alan Lomax | 1:47 | |
| Good Morning Judge | Furry Lewis | 5:31 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Shallow Water | Donald Harrison, Dr. John | 3:03 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Free at Last | Dock Reed, Vera Hall | 1:41 | |
| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Jesse James - Instrumental | Mountain Ramblers | 2:15 | |
| You Better Mind | Bessie Jones | 2:11 | |
| Jubilee | The McIntosh County Shouters | 2:17 | |
| Marie La Veau | Papa Celestin | 6:36 | |
| Dont | Dgreat44 | 1:26 | |
| The Fox and the Hounds | Henry Thomas | 2:36 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| The Lord Will Make A Way | The Fairfield Four | 2:36 | |
| Black Cat Bone | Jessie Mae Hemphill | 4:57 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| The Voice Within | Percy Mayfield | 2:30 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Country Blues (No. 1) - Plantation Recording | Muddy Waters | 3:33 | |