| Blue Yodel No. 4 (California Blues) | Jimmie Rodgers | 3:01 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Swing Low Sweet Chariot | Sister O.M. Terrell | 2:36 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Eu Tive Medo | Quimere, Blind Willie Johnson, Victor Villasanti | 3:40 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Dark Road Blues | Willie Lofton | 3:03 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| Mean Ol' Frisco | Arthur "Big Boy" Crudup | 2:37 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
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| Denver Blues | Tampa Red | 2:54 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| All Around Man | Bo Carter | 3:00 | |
| White House Blues | Charlie Poole | 3:31 | |
| Baby Please Don't Go | Big Joe Williams | 2:44 | |
| Away Out On the Mountain | Jimmie Rodgers | 3:17 | |
| Long Tall Girl Got Stuck on Me | Mance Lipscomb | 2:43 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| I'm Free from the Chain Gang Now | Jimmie Rodgers | 3:02 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Alabama March | J.B. Lenoir | 2:53 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| She Left Me a Mule to Ride | Big Joe Williams | 2:35 | |
| Prodigal Son | Dock Boggs | 3:57 | |
| Police Station Blues | Peetie Wheatstraw | 3:05 | |
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| You Missed a Good Man | Tampa Red | 3:34 | |
| What's the Matter Now? - Remastered | Mississippi Fred McDowell | 4:59 | |
| Little Annie Mae | Big Joe Williams | 2:00 | |
| Match Box Blues | Blind Lemon Jefferson | 3:08 | |
| John Henry | Furry Lewis | 5:49 | |
| A Thousand Miles From Nowhere | Robert Pete Williams | 5:14 | |
| Evil Hearted Woman - Alternate Take | T-Bone Walker | 2:36 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
| My Baby Left Me This Morning | Pink Anderson | 3:38 | |