| Old Country Stomp | Henry Thomas | 2:51 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| The Panama Limited | Bukka White | 3:10 | |
| Denver Blues | Tampa Red | 2:54 | |
| Baby Please Don’t Go | Big Joe Williams | 1:48 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Can't Nobody Hide from God | Blind Willie Johnson | 3:21 | |
| Barbecue Blues | Barbecue Bob | 3:12 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| Paul And Silas In Jail | Washington Phillips | 2:41 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Troubled, Lord I'm Troubled | Bill Landford | 2:53 | |
| My Mama Don't Allow Me | Arthur "Big Boy" Crudup | 3:10 | |
| Frankie and Johnny | Jimmie Rodgers | 2:50 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| Louise McGhee | Son House | 6:11 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| I Am The Light | Rev. Gary Davis | 3:02 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
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| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Take Your Burden To The Lord And Leave It There | Washington Phillips | 3:15 | |
| Pony Blues | Charley Patton | 2:59 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Diddie Wa Diddie | Blind Blake | 3:00 | |
| Sweeter as the Years Roll By | Blind Willie Johnson | 2:46 | |
| Sweet Honey Hole | Blind Boy Fuller | 2:47 | |
| Goodnight, Irene - 2022 Remaster | Lead Belly | 2:32 | |
| You Got To Walk That Lonesome Valley | Rev. F.W. McGee | 3:32 | |
| St. James Infirmary | Josh White | 3:42 | |
| Go to Sleep Little Baby | Bessie Jones | 1:14 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| It Hurts Me Too | Tampa Red | 2:33 | |
| New Orleans (The Rising Sun Blues) | Lead Belly | 3:16 | |
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| He's In The Jailhouse Now | Blind Blake | 2:46 | |
| Levee Camp Moan | Son House | 9:27 | |
| Old Original Kokomo Blues | Kokomo Arnold | 2:54 | |
| Mama Talk To Your Daughter | J.B. Lenoir | 2:21 | |
| Aberdeen Mississippi Blues | Bukka White | 4:10 | |
| Slow Drag (Cincinnati Flow Rag) - Instrumental | Rev. Gary Davis | 2:24 | |
| Hard Time Killing Floor Blues | Skip James | 3:23 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| The Lord Will Make A Way | The Fairfield Four | 2:36 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |