| Milk Cow Blues | Maddox Brothers, Rose Maddox | 3:10 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Sitting on Top of This World | Roscoe Holcomb | 2:42 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Blaze Foley's 113th Wet Dream | Blaze Foley | 3:05 | |
| Where is My Sailor Boy? (What Does the Deep Sea Say?) | Bill Monroe and Doc Watson | 3:31 | |
| Don’t You See That Train | The Delmore Brothers | 3:12 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| John Hardy | Mike Seeger | 2:44 | |
| Ramblin' | Barbara Dane | 2:57 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| I've Endured | Ola Belle Reed, Bud Reed, David Reed | 2:52 | |
| Loch Lomond / Molly Malone | Michael Hurley | 3:06 | |
| Country Blues | Dock Boggs | 3:55 | |
| Salt Creek | Clarence White | 2:39 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Down in the Willow Garden (Rose Connolly) | Iron Mountain String Band | 3:16 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Drunkard's Special | Coley Jones | 3:15 | |
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| I'm Troubled | The Doc Watson Family | 2:42 | |
| Graveyard Blues | Roscoe Holcomb | 2:59 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| In the Pines | The Kossoy Sisters | 3:19 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| The Animal Song - If I Could Ramble Like a Hound | Michael Hurley | 4:44 | |
| Poor Man | Frank Proffitt | 3:02 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Knife Guitar | Roscoe Holcomb | 1:17 | |
| False Love | Paul Clayton | 2:09 | |
| Oh The Wind And Rain | Jody Stecher | 3:49 | |
| High on the Mountain | Ola Belle Reed, David Reed | 3:06 | |
| Cotton Eyed Joe | The New Lost City Ramblers | 1:15 | |
| Home to the Mountains | Betty Fisher | 3:24 | |
| Walking Down the Road | Woody Pines | 3:19 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Engine 143 | The Kossoy Sisters | 4:17 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Satan's Jeweled Crown | The Louvin Brothers | 2:57 | |
| Mining Camp Blues | Hazel Dickens, Alice Gerrard | 3:25 | |
| Old Blue | Furry Lewis | 3:00 | |
| Let Us Get Together Right Down Here | Rev. Gary Davis | 3:09 | |