| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| Cruel War | Iron Mountain String Band | 2:41 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| Broke Down Engine Blues | Blind Willie McTell | 3:11 | |
| Barnyard Banjo Pickin' | Stringbean | 2:18 | |
| Bowling Green | The New Lost City Ramblers, Cousin Emmy | 1:50 | |
| True Love | Roscoe Holcomb | 6:23 | |
| Viola Lee Blues | Cannon's Jug Stompers | 3:10 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Bonaparte's Retreat | Ola Belle Reed, Kevin Roth, Bud Reed | 2:49 | |
| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Raleigh and Spencer/Dusty Miller | Tatiana Hargreaves, Danny Knicely, Emma Beaton, Mark Schatz | 4:05 | |
| Southbound | The Doc Watson Family | 2:40 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Little Pink | Chance McCoy & The Appalachian String Band | 5:02 | |
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| The Daemons Lament/Sugar Baver | Tatiana Hargreaves, Mark Schatz, Sarah Jarosz, Bruce Molsky, Emma Beaton, Danny Knicely | 4:09 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Willie Moore | Burnett & Rutherford | 3:15 | |
| White House Blues | Charlie Poole | 3:31 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| The Girl Who Danced Oklahoma | Terry Allen | 4:18 | |
| Cindy | Frank Proffitt | 2:16 | |
| When First Into this Country | The New Lost City Ramblers | 2:48 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Walkin' in My Sleep | Hazel Dickens, Alice Gerrard | 2:10 | |
| A Home in Old Kentucky | Cousin Emmy, The New Lost City Ramblers | 1:30 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Black Eye Susie | Roscoe Holcomb | 1:14 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| K. C. Railroad Blues | Andrew & Jim Baxter | 3:30 | |
| Riding on That Train 45 | The New Lost City Ramblers | 2:21 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Born and Raised in Covington | Roscoe Holcomb | 2:16 | |
| Let's Get Drunk Again | Sam Chatmon | 2:18 | |
| Hog of the Foresaken | Michael Hurley | 4:35 | |
| Hello Stranger | Any Old Time String Band | 2:18 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| John Henry | Big Bill Broonzy | 4:44 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Mining Camp Blues | Hazel Dickens, Alice Gerrard | 3:25 | |
| Cluck Old Hen | Dirk Powell, John Hermann, Tim O'Brien | 2:03 | |
| Lorene | The Louvin Brothers | 2:03 | |
| Hard Time Killing Floor Blues | Skip James | 3:28 | |