| Roving Gambler | The Country Gentlemen | 3:10 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| Pretty Saro | Hedy West | 3:40 | |
| Get Thee Behind Me, Satan | The Almanac Singers | 2:36 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Turkey Reveille | Barbara Dane | 4:45 | |
| Ruthie | Frank Fairfield | 3:39 | |
| Railroad Bill - Remastered | Hobart Smith | 2:42 | |
| John Hardy | Frank Proffitt | 2:27 | |
| Little Sadie | Doc Watson | 2:22 | |
| Boat's Up the River | Roscoe Holcomb | 3:50 | |
| Nine Pound Hammer | Frank Fairfield | 3:57 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Wild Bill Jones | Dock Boggs | 2:14 | |
| Dark Holler Blues | Clarence Ashley | 2:58 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| A Home in Old Kentucky | Cousin Emmy, The New Lost City Ramblers | 1:30 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Johnny Booker | The New Lost City Ramblers, Cousin Emmy | 1:32 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
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| Graveyard Blues | Roscoe Holcomb | 3:22 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| Jerry, Go Oil That Car | Harry McClintock | 2:40 | |
| Salt Creek | Clarence White | 2:39 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Piney Mountains | Bruce Molsky | 3:10 | |
| Drunkard's Special | Coley Jones | 3:15 | |
| Poor Man | Frank Proffitt | 3:02 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Down the Road | The Doc Watson Family | 1:41 | |
| I Will Always Be Waiting For You | Jim & Jesse | 2:14 | |
| East Virginia Blues | Roscoe Holcomb | 4:50 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Lazy John | Chance McCoy & The Appalachian String Band | 2:59 | |
| Coal Creek March | Pete Steele | 1:27 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Hog of the Foresaken | Michael Hurley | 4:35 | |
| Foggy Mountain Top | Bill Monroe and Doc Watson | 2:46 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Billy in the Low Ground | Clarence White | 1:37 | |
| Lord, I Feel Just Like Goin' On | Rev. Gary Davis | 3:30 | |
| Goodbye Blues | Blind Willie McTell | 1:39 | |
| House Of The Risin' Sun - Rising Sun Blues | Clarence Ashley | 2:39 | |