| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Banks of the Ohio | Bill Monroe and Doc Watson | 3:33 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:05 | |
| Wild Bill Jones | Frank Proffitt | 3:27 | |
| Rock About Saro Jane | Uncle Dave Macon | 3:23 | |
| Last Chance | Hobart Smith | 1:30 | |
| Where is My Sailor Boy? (What Does the Deep Sea Say?) | Bill Monroe and Doc Watson | 3:31 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Reuben's Train | Foghorn Stringband | 5:03 | |
| Frankie and Johnny | Roscoe Holcomb | 5:07 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Crow Jane | Etta Baker, Cora Phillips | 2:21 | |
| Knife Guitar | Roscoe Holcomb | 1:17 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Suicide Blues | Stringbean | 2:26 | |
| Southbound | The Doc Watson Family | 2:40 | |
| Bonaparte's Retreat | Ola Belle Reed, Kevin Roth, Bud Reed | 2:49 | |
| Sugar Hill | Iron Mountain String Band | 3:39 | |
| The Little Glass Of Wine | Stanley Brothers | 3:05 | |
| Jaybird March | Etta and Cora Baker | 1:50 | |
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| The Panama Limited | Bukka White | 3:10 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Lone Prairie | Wade Ward | 0:56 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Boat's Up the River | Roscoe Holcomb | 3:50 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| God Don't Never Change | Blind Willie Johnson | 2:56 | |
| Hog of the Foresaken | Michael Hurley | 4:35 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| John Henry | Furry Lewis | 4:51 | |
| Foggy Mountain Top | Bill Monroe and Doc Watson | 2:46 | |
| Walking Down the Road | Woody Pines | 3:19 | |
| The Butcher Boy | Peggy Seeger | 2:20 | |
| Oh The Wind And Rain | Jody Stecher | 3:49 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Johnny Booker | The New Lost City Ramblers, Cousin Emmy | 1:32 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Frog Went A-Courtin' | Almeda Riddle | 2:48 | |
| Trouble Will Soon Be Over | Blind Willie Johnson | 3:06 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| House Of The Risin' Sun - Rising Sun Blues | Clarence Ashley | 2:39 | |
| Half Shaved / High Atmosphere | Wade Ward | 2:11 | |
| Let Us Get Together Right Down Here | Rev. Gary Davis | 3:09 | |
