| Black Eye Susie | Roscoe Holcomb | 1:14 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| In the Pines | The Kossoy Sisters | 3:19 | |
| Poor Boy | Harry McClintock | 2:14 | |
| K. C. Railroad Blues | Andrew & Jim Baxter | 3:30 | |
| Frazier Blues | Frank Fairfield | 4:21 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| When First Into this Country | The New Lost City Ramblers | 2:48 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Broke Down Engine Blues | Blind Willie McTell | 3:11 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Long Haired Preachers (Preacher and the Slave) | Harry McClintock | 3:42 | |
| Hiram Hubbard | Jean Ritchie and Doc Watson | 3:50 | |
| The Little Glass Of Wine | Stanley Brothers | 3:05 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Jaybird March | Etta and Cora Baker | 1:50 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
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| How Can a Poor Man Stand Such Times and Live | The New Lost City Ramblers | 3:35 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Darling Cory | Roscoe Holcomb | 3:42 | |
| I've Endured | Ola Belle Reed, Bud Reed, David Reed | 2:52 | |
| Goin' Down This Road | Big Bill Broonzy | 2:22 | |
| Sugar Hill | Mary Z. Cox | 3:33 | |
| Pig Meat Is What I Crave | Bo Carter | 2:51 | |
| John Hardy | Mike Seeger | 2:44 | |
| The Old lady & the devil | Bill & Belle Reed | 3:06 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| Ramblin' Blues | Charlie Poole | 3:07 | |
| Coney Isle | Roscoe Holcomb | 1:52 | |
| Motherless Children Have a Hard Time | Blind Willie McTell | 2:56 | |
| Kingdom Come | Norman Edmonds | 2:06 | |
| Your Long Journey | The Doc Watson Family | 2:36 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| A Home in Old Kentucky | Cousin Emmy, The New Lost City Ramblers | 1:30 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |
| Old Blue | Furry Lewis | 3:00 | |
| Broke Down Engine Blues | Blind Willie McTell | 2:45 | |
| Washington D.C. Hospital Center Blues | Skip James | 4:11 | |
| Get Down On Your Knees And Pray | The Kentucky Colonels | 3:09 | |
| Things In Life | Don Stover | 3:07 | |
| Twin Sisters | Sidna Myers | 0:57 | |