| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| Chasin' Rainbows | R. Crumb And His Cheap Suit Serenaders | 3:17 | |
| On the Banks of the Ohio - From Paper Moon | The Blue Sky Boys | 3:20 | |
| Blue Ghost Blues | Lonnie Johnson | 3:09 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| Dry Southern Blues | Blind Lemon Jefferson | 2:53 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Don’t You See That Train | The Delmore Brothers | 3:12 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Railroad Blues | Sam McGee | 2:48 | |
| You're Biscuits Are Big Enough for Me | Bo Carter | 2:07 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| Buck Dance - Version; Instrumental; Remastered | Mance Lipscomb | 1:33 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
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| Cindy | Frank Proffitt | 2:16 | |
| Little Birdie | Roscoe Holcomb | 2:28 | |
| Alabama Bound - Version; Take 2; Remastered | Mance Lipscomb | 2:36 | |
| John Henry | Henry Thomas | 2:46 | |
| Miss the Mississippi and You | Jimmie Rodgers | 2:55 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| Willie Moore | Burnett & Rutherford | 3:15 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Tell It To Me | Grant Brothers & Their Music | 2:56 | |
| Shake 'Em On Down | Bukka White | 3:00 | |
| Bull Doze Blues | Henry Thomas | 3:28 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| A Lazy Farmer Boy | Buster Carter & Preston Young | 3:01 | |
| Mississippi County Farm Blues | Son House | 2:50 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Bonaparte's Retreat | Ola Belle Reed, Kevin Roth, Bud Reed | 2:49 | |
| Long Lonesome Road | Peggy Seeger | 2:17 | |
| Swing Low Sweet Chariot | Sister O.M. Terrell | 2:36 | |
| Crawdad Hole | Jessie Mae Hemphill | 2:07 | |
| Hey, Hey Baby | The Holy Modal Rounders | 1:20 | |
| Hard Time Killing Floor Blues | Skip James | 3:23 | |
| John Henry | Furry Lewis | 5:49 | |
| Blues In The Bottle | The Holy Modal Rounders | 3:24 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| Old Blue | Furry Lewis | 3:00 | |
| Fly Around My Pretty Little Miss | Ola Belle Reed | 2:00 | |
| Devil Got My Woman | Skip James | 5:15 | |