| Mashing That Thing | Bo Carter | 2:40 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Hot As I Am | Saddle Tramps | 2:18 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Give Me Back My Five Dollars | Uncle Dave Macon | 2:53 | |
| Old Nevada Moon | Patsy Montana | 2:56 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Pipeliner Blues | Moon Mullican | 2:32 | |
| No Depression in Heaven | The New Lost City Ramblers | 3:00 | |
| Cool Water | Sons of the Pioneers | 2:09 | |
| Sail Away Ladies | Uncle Dave Macon | 2:58 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Cocaine | Dick Justice | 3:12 | |
| Muleskinner Blues | Grandpa Jones | 4:10 | |
| Train on the Island | Hazel Dickens, Alice Gerrard | 1:44 | |
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| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| The Farmer's Curst Wife | Estil C. Ball | 2:59 | |
| Praise God I'm Satisfied | Blind Willie Johnson | 3:10 | |
| I Wanna Be A Cowboy's Sweetheart (Alternate Take) | Patsy Montana | 3:08 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| You Can't Get That Stuff No More | Tampa Red, Georgia Tom | 2:56 | |
| Oh Molly Dear | B.F. Shelton | 3:00 | |
| My Baby's Just Like Money - 1950 Version | Lefty Frizzell | 2:19 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:05 | |
| I'm Bound to Follow the Longhorn Cows | Alan Lomax | 3:43 | |
| Broke Down Engine Blues | Blind Willie McTell | 3:11 | |
| Willow Garden | Roscoe Holcomb | 3:27 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Pistol Packin' Mama | Al Dexter | 2:47 | |
| John Hardy | Mike Seeger | 2:44 | |
| Rattlesnake | Alan Lomax | 1:34 | |
| Sunny Side Of The Mountain | Jimmy Martin | 2:40 | |
| So Long to the Red River Valley | Johnny Bond | 2:05 | |
| I Ain't Got Nobody | Dick Curless | 2:00 | |
| Man In Love | Charlie Feathers | 1:55 | |
| The Green Rolling Hills Of West Virginia | Hazel Dickens, Alice Gerrard | 3:24 | |
| A Satisfied Mind | Jean Shepard | 2:28 | |
| I Got Texas In My Soul | Tex Williams | 2:57 | |
| Brilliancy Medley | Eck Robertson | 2:57 | |
| Shall We Gather At The River | Uncle Dave Macon | 3:29 | |
| Two Whoops And A Holler | Jean Shepard | 2:21 | |
| I Heard The Jukebox Playing | Kitty Wells | 2:20 | |
| Get Along, Little Dogies | Tex Ritter | 3:05 | |