| Lee Highway Blues | Hazel Dickens, Alice Gerrard | 1:42 | |
| Last Payday at Coal Creek | Pete Steele | 3:03 | |
| Rose of Sharon | Bobby Fullbright, Charles Summer, J.C. Owens, Jimmy Pruitt, Nick Hallman, Paul Gregory, TC Foster, Ted Brackett, Vernon Riddle | 2:18 | |
| Little Birdie | J.D. Wilkes | 3:15 | |
| Goodbye Old Paint | Harry McClintock | 2:49 | |
| Calvary | Dock Boggs | 3:33 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Hills of Mexico | J.D. Wilkes | 2:59 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Reuben Train | Frank Proffitt | 2:57 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| When Can I Change My Clothes? | Bukka White | 2:59 | |
| Unwelcomed Guest | The Younghearts | 2:55 | |
| I Am a Pilgrim | Clarence White | 1:39 | |
| New Prisoner's Song | Dock Boggs | 2:57 | |
| Don't Let Your Deal Go Down Blues | Charlie Poole with the North Carolina Ramblers | 2:53 | |
| Down in the Willow Garden (Rose Connolly) | Iron Mountain String Band | 3:16 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Bluegrass Banjo | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:41 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
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| Lonesome Yodel Blues | The Delmore Brothers | 2:37 | |
| Twinkle, Twinkle, Little Star | Snuffy Jenkins, Homer "Pappy" Sherrill | 1:18 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Movin' on Down the River - Remastered | Jean Ritchie | 2:23 | |
| Piney Mountains | Bruce Molsky | 3:10 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| Coal Creek | Roscoe Holcomb | 1:31 | |
| Oh, Death | Dock Boggs | 3:20 | |
| Muleskinner Blues | Grandpa Jones | 4:10 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| The Lone Star Trail | Ken Maynard | 3:12 | |
| Pretty Little Widow | Stringbean | 1:28 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| Gypsum Davy | Jean Ritchie | 1:56 | |
| The Devil's Question | Paul Clayton, Jean Ritchie, Richard Chase | 2:29 | |
| Betty Baker | Chance McCoy & The Appalachian String Band | 2:50 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Give Me a Heart to Love | Rev. Gary Davis | 2:24 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| Sally Ann | The Kentucky Colonels | 1:36 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| I Could Change My Mind | The Johnson Mountain Boys | 1:48 | |
