| T.B. Blues (They're at Rest Together) | Hazel Dickens, Alice Gerrard | 3:28 | |
| East Tennessee Blues | Bill Monroe and Doc Watson | 2:12 | |
| The Devil's Dream | Blind Boy Paxton | 3:25 | |
| Piney Mountains | Bruce Molsky | 3:10 | |
| Don't Sing Love Songs | Barbara Dane | 4:00 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Kentucky Mandolin | Bill Monroe and Doc Watson | 1:59 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Knife Guitar | Roscoe Holcomb | 1:17 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Denomination Blues, Pt. 1 | Washington Phillips | 3:07 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| Good Morning Judge | Furry Lewis | 5:31 | |
| Banks of the Ohio | Bill Monroe and Doc Watson | 3:33 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| Ida Red | Anna & Elizabeth | 1:58 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
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| Talking Banjo | Buzz Busby | 1:39 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Cripple Creek | David Johnson | 2:11 | |
| Walkin' in My Sleep | Hazel Dickens, Alice Gerrard | 2:10 | |
| Prodigal Son | Dock Boggs | 3:57 | |
| Bowling Green | The Kossoy Sisters | 2:13 | |
| Reuben Train | Frank Proffitt | 2:57 | |
| Mole in the Ground | Karen Dalton | 3:29 | |
| Hills of Mexico | Nora Brown | 4:10 | |
| Leatherwing Bat | Peggy Seeger | 2:11 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| Bury Me Beneath the Willow | Clarence White | 1:56 | |
| Coal Miner's Blues - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:42 | |
| Going Across the Mountain | Frank Proffitt | 2:16 | |
| Sally Ann | Riley Baugus | 1:20 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Waiting for a Train | Jimmie Rodgers | 2:47 | |
| Johnny Booker | The New Lost City Ramblers, Cousin Emmy | 1:32 | |
| The Coo-Coo Bird | Doc Watson | 2:36 | |
| Lorene | The Louvin Brothers | 2:03 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Kill It Kid - Bonus Track | Blind Willie McTell | 2:31 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Forkey Deer | Sidna Myers | 2:05 | |
| Reconciled To The Blues | Michael Hurley | 3:02 | |
| Someday Soon | Ian & Sylvia | 2:16 | |