| Banana in Your Fruit Basket | Bo Carter | 3:08 | ||
| Kissing in the Dark | Memphis Minnie | 2:31 | ||
| Levee Camp Moan | Son House | 9:27 | ||
| Breadline Blues | The New Lost City Ramblers | 2:37 | ||
| Dope Head Blues | Victoria Spivey | 3:15 | ||
| Seminole Blues | Tampa Red | 3:05 | ||
| Aunt Caroline Dyer Blues | Memphis Jug Band | 3:06 | ||
| Worried Now, Won't Be Worried Long - Previously Unreleased | Sidney Carter, Alan Lomax | 2:55 | ||
| Little Birdie | Roscoe Holcomb | 2:28 | ||
| Bottleneck Blues | Sylvester Weaver | 2:56 | ||
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | ||
| Cocaine | Dick Justice | 3:12 | ||
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | ||
| Charmin' Betsy | Henry Thomas | 3:01 | ||
| Willie Moore | Burnett & Rutherford | 3:15 | ||
| Levee Camp Blues | Son House | 3:45 | ||
| John the Revelator | Blind Willie Johnson | 3:16 | ||
| Devil Sent the Rain Blues | Charley Patton | 3:05 | ||
| Frog Went A-Courtin' | Almeda Riddle | 2:48 | ||
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | ||
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | ||
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| Henry Lee (Original Mix) | Dick Justice | 3:25 | ||
| Hoodoo Lady | Memphis Minnie | 3:02 | ||
| A Lazy Farmer Boy | Buster Carter & Preston Young | 3:01 | ||
| Parchman Farm Blues | Bukka White | 2:42 | ||
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | ||
| I Want Jesus to Walk with Me | James Shorty | 3:43 | ||
| Buck Dance - Version; Instrumental; Remastered | Mance Lipscomb | 1:33 | ||
| Sweet Honey Hole | Blind Boy Fuller | 2:47 | ||
| Railroad Bill - Remastered | Hobart Smith, Alan Lomax | 2:42 | ||
| Mississippi Bottom Blues | Kid Bailey | 2:49 | ||
| Sun Brimmer's Blues | Memphis Jug Band | 3:12 | ||
| Guitar Rag | Sylvester Weaver | 3:06 | ||
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | ||
| County Farm Blues | Son House | 2:12 | ||
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | ||
| Terraplane Blues | Robert Johnson | 2:58 | ||
| Ragged And Dirty | William Brown | 4:50 | ||
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | ||
| Rock Me Mama | Arthur "Big Boy" Crudup | 2:29 | ||
| Let's Get Drunk Again | Sam Chatmon | 2:18 | ||
| Someday Baby Blues | Sleepy John Estes | 2:59 | ||
| Some Day Baby | Lonnie Johnson | 3:08 | ||
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | ||
| Blue As I Can Be | Tommy McClennan | 2:42 | ||
| If the River Was Whiskey | Charlie Poole | 3:10 | ||
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | ||
| Graveyard Blues | Robert Pete Williams | 2:46 | ||
| When My Baby Left Me | Furry Lewis | 3:23 | ||
