| Ruby | Smokey River Boys | 3:02 | |
| Pine Grove Blues | Nathan Abshire | 2:59 | |
| Hell Hound On My Trail | Robert Johnson | 2:37 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Roll Jordan Roll (feat. Chiwetel Ejiofor & Cast) | Topsy Chapman, Chiwetel Ejiofor, Cast | 1:57 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Ain't It Grand to Live a Christian Life | Blind Willie McTell | 2:38 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Pig Meat Is What I Crave | Bo Carter | 2:51 | |
| Cross Road Blues - Take 1 | Robert Johnson | 2:37 | |
| San Francisco Bay Blues | Jesse Fuller | 3:28 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| How Long | C.W. Stoneking | 3:13 | |
| Fox Chase | Deford Bailey | 3:01 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Ain't No Grave | The Crooked Jades | 3:26 | |
| Jim Jones at Botany Bay | Gary Shearston | 3:10 | |
| Let's Disagree | Leroy Carr | 2:56 | |
| Devil In The Bayou | Harry Choates | 2:13 | |
| Hallelujah, I'm a Bum | Harry McClintock | 2:57 | |
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| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| That's All Right | Arthur "Big Boy" Crudup | 2:53 | |
| Joe Lee's Rock | Boy Blue | 3:36 | |
| Those Gambler's Blues | Jimmie Rodgers | 2:49 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Early In The Mornin' | C.W. Stoneking | 3:36 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Southern Can Is Mine | Blind Willie McTell | 3:13 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Talking Banjo | Buzz Busby | 1:39 | |
| Can't You Line 'Em | James Wilson, R. Ramsay, George Goram, R. Brown, J. Kirby, Lemuel Jones, C. Meekins, Ed Lewis | 1:53 | |
| Crazy Blues | Mamie Smith | 3:22 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Tiger Man (King of the Jungle) | Rufus Thomas | 2:48 | |
| He's Been A Shelter For Me | The Soul Stirrers | 2:51 | |
| Big Rock Candy Mountain | Norman Blake | 2:18 | |
| Louisiana Blues | Muddy Waters | 2:54 | |
| Early In The Mornin' | 22, Little Red, Tangle Eye, Hard Hair | 4:42 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Lucky Holler | Ed Lewis | 2:28 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |