| Ramblin' On My Mind | Robert Johnson | 2:52 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Follow the Drinking Gourd | Richie Havens | 3:23 | |
| I'm a Rattlesnakin' Daddy | Blind Boy Fuller | 3:04 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Old Dollar Mamie | Alan Lomax | 3:24 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Cross Road Blues - Take 2 | Robert Johnson | 2:27 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Davidson County Blues - Remastered 2002 | Deford Bailey | 3:20 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Where the Lemon Trees Bloom | Susan Kanzian, Ed Lewis | 2:26 | |
| Till The Cows Come Home | Lucille Bogan | 2:52 | |
| When the Sun Goes Down | Leroy Carr | 2:59 | |
| Green Sally, Up | Jesse Lee Pratcher & Mattie Garder | 0:43 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Slow Down | J.B. Lenoir | 1:34 | |
| I Got the Cross the River Jordan | Blind Willie McTell | 4:00 | |
| Eyes On The Prize ("Hold On") - Voice | Traditional, Sweet Honey In The Rock, James Horner | 3:35 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
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| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Roll Jordan Roll (feat. Chiwetel Ejiofor & Cast) | Topsy Chapman, Chiwetel Ejiofor, Cast | 1:57 | |
| Just Like a Bird Without a Feather | Samuel L. Jackson | 2:22 | |
| Which Side are You On? | The Almanac Singers | 2:11 | |
| The Preacher and the Bear | Jubalaires | 2:49 | |
| My Girl's Pussy | Harry Roy & His Bat Boys Club, Harry Roy and His Orchestra | 3:12 | |
| On That Rock | Viola James | 2:56 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Free at Last | Dock Reed, Vera Hall | 1:41 | |
| Dirtiest Little Darling | Blind Boy Paxton, NO | 3:31 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Get 'Em From The Peanut Man (Hot Nuts) | Lil Johnson | 3:10 | |
| Get Thee Behind Me, Satan | The Almanac Singers | 2:36 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| If the River Was Whiskey | Charlie Poole | 3:10 | |
| Downtown Blues | Frank Stokes | 3:17 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Allons รก Lafayette | Joe Falcon, Cleoma Breaux | 2:56 | |
| A Conversation With Death | Lloyd Chandler | 4:10 | |
| Graveyard Blues | Robert Pete Williams | 2:46 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Come And Go With Me To That Land | Bernice Johnson Reagon | 4:40 | |
| Ezekiel Saw The Wheel | The Dixie Hummingbirds | 1:33 | |
| Tiger Man (King of the Jungle) | Rufus Thomas | 2:48 | |