| Rowdy Blues | Kid Bailey | 3:00 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Hellhound on My Trail | Robert Johnson | 2:34 | |
| My Trouble Is Hard | Jubalaires | 2:05 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Pretty Polly | Dock Boggs | 2:56 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| High on a Mountain | Ola Belle Reed | 5:30 | |
| Downtown Blues | Frank Stokes | 3:17 | |
| Trucking My Blues Away No 2 | Blind Boy Fuller | 2:55 | |
| Blow, Gabriel | The McIntosh County Shouters | 3:37 | |
| Lonesome Blues | Alan Lomax | 3:29 | |
| Follow the Drinking Gourd | Richie Havens | 3:23 | |
| Hoodoo Man Blues | Junior Wells, Buddy Guy | 2:06 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| How Long, How Long Blues | Leroy Carr | 3:03 | |
| Fox Chase | Deford Bailey | 3:01 | |
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| Marie La Veau | Papa Celestin | 6:36 | |
| Crawlin' King Snake | John Lee Hooker | 3:02 | |
| Cross Road Blues - Take 2 | Robert Johnson | 2:27 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Troubled, Lord I'm Troubled | Bill Landford | 2:53 | |
| Eyes On The Prize ("Hold On") - Voice | Traditional, Sweet Honey In The Rock, James Horner, Aisha Kahlil | 3:35 | |
| Live A-Humble | Jubalaires | 2:35 | |
| That's All Right | Arthur "Big Boy" Crudup | 2:53 | |
| The Little Old Log Cabin In The Lane | Fiddlin John Carson | 2:47 | |
| I'll Be So Glad When the Sun Goes Down - Remastered | Ed Young, Unidentified prisoners, Alan Lomax | 5:01 | |
| The Unfortunate Rake | A. L. Lloyd and Alf Edwards | 2:59 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| I Got a Woman Cross Town | Pink Anderson | 1:54 | |
| It Makes a Long Time Man Feel Bad | Alan Lomax | 2:40 | |
| Don't Ease Me In | Henry Thomas | 2:58 | |
| Traveling Riverside Blues | Robert Johnson | 2:47 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Kenny Wagner | Bob Carpenter | 2:34 | |
| Which Side are You On? | The Almanac Singers | 2:11 | |
| Blind Arthur's Breakdown | Blind Blake | 3:00 | |
| Standing In My Doorway Crying | Jessie Mae Hemphill | 4:40 | |
| Come And Go With Me To That Land | Bernice Johnson Reagon | 4:40 | |
| She Screams (feat. Tish Songbird) | Ed Lewis, Tish Song Bird | 3:00 | |
| Ezekiel Saw The Wheel | The Dixie Hummingbirds | 1:33 | |
| Come, Let's Go Back to God - Remastered | The Soul Stirrers | 2:48 | |
| Goodbye Blues | Blind Willie McTell | 1:39 | |
| Lucky Holler | Ed Lewis | 2:28 | |
| O Death | Ralph Stanley | 3:19 | |
