| The Little Red Caboose | Henry Thomas | 2:47 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| What's That Smells Like Fish | Blind Boy Fuller | 2:46 | |
| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| Across the Rocky Mountain | Roscoe Holcomb | 5:22 | |
| Shady Grove | The Doc Watson Family | 2:17 | |
| Cottonfield Blues | Henry Thomas | 2:46 | |
| Franklin Roosevelt's Back Again | The New Lost City Ramblers | 2:21 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| I'm so Glad | Skip James | 2:49 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Bull Doze Blues | Various Artists, Henry Thomas | 3:28 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Rockin Chair Blues | Robert Petway | 3:01 | |
| If the River Was Whiskey | Charlie Poole | 3:10 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Let's Get Drunk Again | Sam Chatmon | 2:18 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Red River Blues | Henry Thomas | 3:04 | |
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| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Mississippi Delta Blues | Leon Redbone | 1:45 | |
| Aunt Caroline Dyer Blues | Memphis Jug Band | 3:06 | |
| Don't Let Your Deal Go Down Blues | Charlie Poole with the North Carolina Ramblers | 2:53 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Black Cat Blues | Memphis Minnie | 2:54 | |
| Fallin' Down Blues | Robert Wilkins | 2:38 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Levee Camp Blues | Son House | 3:45 | |
| T.B. Blues | Jimmie Rodgers | 2:58 | |
| Oh Molly Dear | B.F. Shelton | 3:00 | |
| Drop Down Mama | Sleepy John Estes | 3:11 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Indian War Whoop | Floyd Ming & His Pep Steppers | 3:08 | |
| Charmin Betsey | Henry Thomas | 3:03 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Poor Man | Frank Proffitt | 3:02 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Cocaine | Dick Justice | 3:12 | |
| Wayward Girl Blues | Lottie Kimbrough, Milas Pruitt | 2:52 | |
| Barbecue Blues | Barbecue Bob | 3:12 | |
| Coal Miner's Blues - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:42 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |