| Good Biscuits | Memphis Minnie | 2:40 | |
| Born Dead | J.B. Lenoir | 3:47 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| That's No Way To Do | Pink Anderson | 2:23 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| Whistlin' Pines | Big Joe Williams | 2:57 | |
| Weeping Willow Blues | Bessie Smith | 3:09 | |
| Bye and Bye I'm Goin' to See the King | Blind Willie Johnson | 2:51 | |
| New Orleans (The Rising Sun Blues) | Lead Belly | 3:16 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| Po' Boys | Sonny Terry and Brownie McGhee | 2:43 | |
| Reefer Head Woman | Jazz Gillum | 3:03 | |
| It's Nobody's Fault but Mine | Blind Willie Johnson | 3:08 | |
| Dope Head Blues | Victoria Spivey | 3:15 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Diddie Wa Diddie | Blind Blake | 3:00 | |
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| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| School Boy | Big Mama Thornton | 4:30 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| West Coast Blues | Blind Blake | 3:08 | |
| Milwaukee Blues | Charlie Poole | 3:18 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Someday Baby Blues | Sleepy John Estes | 2:59 | |
| Louise | Mississippi Fred McDowell | 3:58 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Dead Drunk Blues | Ma Rainey | 2:52 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Good Morning School Girl - 2002 Remastered | Sonny Boy Williamson I | 2:58 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Bye-Bye Blues | Tommy Johnson | 3:04 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| That Black Snake Moan | Blind Lemon Jefferson | 2:59 | |
| Goin' Away Baby - Single Version | Jimmy Rogers | 2:46 | |
| Slow Drag (Cincinnati Flow Rag) - Instrumental | Rev. Gary Davis | 2:24 | |
| High And Lonesome | Jimmy Reed | 2:47 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Baby Please Don't Go | Pink Anderson | 2:45 | |
| Old Blue | Furry Lewis | 3:00 | |