| When the Train Comes Along | Henry Thomas | 2:56 | |
| Friar's Point Blues | Robert Nighthawk | 2:54 | |
| Pistol slappin' blues | Blind Boy Fuller | 2:39 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Let's Disagree | Leroy Carr | 2:56 | |
| One Dime Blues | Blind Lemon Jefferson | 2:48 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| White House Blues | Charlie Poole | 3:31 | |
| Mean Old Bedbug Blues | Furry Lewis | 2:45 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Alabama March | J.B. Lenoir | 2:53 | |
| Cottonfield Blues, Pt. 2 | Garfield Akers | 3:21 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Got The Bottle Up And Gone | Sonny Boy Williamson I | 2:33 | |
| Big Fat Mama | David "Honeyboy" Edwards | 2:58 | |
| All I Want Is a Spoonful | Papa Charlie Jackson | 2:42 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Rocky Road | Alabama Sacred Harp Singers | 2:46 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| Denver Blues | Tampa Red | 2:54 | |
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| Old Country Stomp | Henry Thomas | 2:51 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Old Original Kokomo Blues | Kokomo Arnold | 2:54 | |
| Take Your Stand | Blind Willie Johnson | 3:00 | |
| That's All Right, Pt. 1 | John Henry Barbee | 3:40 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Preachin' Blues | Son House | 5:43 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Bugger Burns | Spider John Koerner, Dave Ray, Tony Glover | 1:40 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| Bye and Bye I'm Goin' to See the King | Blind Willie Johnson | 2:51 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| Linin' Track | Spider John Koerner, Dave Ray, Tony Glover | 2:19 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Blues Before Sunrise | Scrapper Blackwell | 5:08 | |
| Preachin' Blues | Son House | 5:16 | |
| Slow Drag (Cincinnati Flow Rag) - Instrumental | Rev. Gary Davis | 2:24 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Let Us Get Together Right Down Here | Rev. Gary Davis | 3:09 | |
| Goodbye Blues | Blind Willie McTell | 1:39 | |
| That Black Snake Moan | Blind Lemon Jefferson | 2:59 | |
