| Down South Blues | Dock Boggs | 2:11 | |
| Moaning the Blues | Memphis Minnie | 3:06 | |
| Dark Was the Night | Blind Willie Johnson | 3:22 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Southern Can Is Mine | Blind Willie McTell | 3:13 | |
| Friar's Point Blues | Robert Nighthawk | 2:54 | |
| Mississippi Boweavil Blues | Charley Patton | 3:11 | |
| Railroad Blues | Sam McGee | 2:48 | |
| I'm Gonna Cross the River Of Jordan | Blind Willie Johnson | 3:05 | |
| Down By The Riverside | Sonny Terry and Brownie McGhee | 3:16 | |
| Aunt Caroline Dyer Blues | Memphis Jug Band | 3:06 | |
| Bandit Cole Younger | Edward L. Crain | 2:53 | |
| Bugger Burns | Spider John Koerner, Dave Ray, Tony Glover | 1:40 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Let's Disagree | Leroy Carr | 2:56 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| It's Tight Like That | Tampa Red | 2:49 | |
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| Captain, Captain - Version; Take 2; Remastered | Mance Lipscomb | 2:34 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Mean Old Frisco Blues - Remastered 2003 | Arthur "Big Boy" Crudup | 2:35 | |
| She Left Me a Mule to Ride | Big Joe Williams | 2:35 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| Georgia Bound | Blind Blake | 3:22 | |
| Hangman | Spider John Koerner, Dave Ray, Tony Glover | 2:32 | |
| Georgia Rag | Blind Willie McTell | 3:03 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| Rockin Chair Blues | Robert Petway | 3:01 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Cocaine Done Killed My Baby | Mance Lipscomb | 1:23 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| New Orleans (The Rising Sun Blues) | Lead Belly | 3:16 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Pistol slappin' blues | Blind Boy Fuller | 2:39 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Stack-O-Lee | Champion Jack Dupree | 3:56 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| Can't Nobody Hide from God | Blind Willie Johnson | 3:31 | |
| Blues Before Sunrise | Scrapper Blackwell | 5:08 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Mother Earth | Memphis Slim | 2:39 | |
| Evil Hearted Woman - Alternate Take | T-Bone Walker | 2:36 | |