| The Twelves (The Dirty Dozens) | Casey Bill Weldon, Kokomo Arnold | 3:11 | |
| Frankie and Johnny | Jimmie Rodgers | 2:50 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| Wild Bill Jones | Dock Boggs | 2:11 | |
| Oh Death | Charley Patton | 2:52 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| Bill Morgan and His Gal (My Name is Morgan But it Ain't J.P. ) | The New Lost City Ramblers | 2:58 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Prayer of a Miner's Child | Dock Boggs | 3:21 | |
| Shady Grove | The Doc Watson Family | 2:17 | |
| Let Your Light Shine on Me | Blind Willie Johnson | 3:08 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Bye Bye Baby Blues | Texas Alexander | 3:09 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| The Cuckoo Bird | The Doc Watson Family | 3:03 | |
| Wild Bill Jones | Frank Proffitt | 3:27 | |
| Poor Boy | Harry McClintock | 2:14 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Keep Your Lamp Trimmed And Burning | Blind Willie Johnson | 3:02 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| Untrue Blues | Blind Boy Fuller | 2:49 | |
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| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Nine Pound Hammer | Ola Belle Reed, John Coffey, David Reed, Bud Reed | 3:25 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Down the Road | The Doc Watson Family | 1:41 | |
| One Dime Blues | Blind Lemon Jefferson | 2:48 | |
| Tell It To Me | Grant Brothers & Their Music | 2:56 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| Drunkard's Lone Child | Dock Boggs | 4:08 | |
| Go Dig My Grave | Jean Ritchie and Doc Watson | 3:50 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Mississippi Boweavil Blues | Charley Patton | 3:11 | |
| Skin Game Blues | Peg Leg Howell | 3:05 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Cindy | Frank Proffitt | 2:16 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| I've Endured | Ola Belle Reed, David Reed, John Coffey, Bud Reed | 2:40 | |
| Danville Girl | Dock Boggs | 2:39 | |
| Tom Rushen Blues | Charley Patton | 3:07 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |