| My Old Horse Died | Dock Boggs | 1:47 | |
| Mississippi County Farm Blues | Son House | 2:50 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| Go Dig My Grave | Jean Ritchie and Doc Watson | 3:50 | |
| Rambling Hobo | The Doc Watson Family | 1:38 | |
| I've Endured | Ola Belle Reed, David Reed, John Coffey, Bud Reed | 2:40 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| Wildwood Flower | Kilby Snow | 1:26 | |
| I'm A Bad Luck Woman | Memphis Minnie | 3:00 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| I Saw the Light | Ola Belle Reed, John Coffey, Bud Reed, David Reed, Alex Campbell | 4:56 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Turkey Reveille | Barbara Dane | 4:45 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Story about singing Rowan County Crew | Dock Boggs | 1:31 | |
| Nobody's Business | Riley Puckett | 2:40 | |
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| Springtime of Life | Ola Belle Reed, Bud Reed, David Reed | 4:22 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Mean Old Bedbug Blues | Furry Lewis | 2:45 | |
| Engine 143 | The Kossoy Sisters | 4:17 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| Kentucky Bootlegger | The New Lost City Ramblers | 2:09 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| One Dime Blues | Blind Lemon Jefferson | 2:48 | |
| Praise God I'm Satisfied | Blind Willie Johnson | 3:10 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| Oh, Death | Dock Boggs | 3:20 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Don't Sing Love Songs | Barbara Dane | 4:00 | |
| I've Endured | Ola Belle Reed, Bud Reed, David Reed | 2:52 | |
| Single Girl | The Kossoy Sisters | 2:16 | |
| John Henry | Henry Thomas | 2:43 | |
| Go Ahead, Buddy | Casey Bill Weldon, Kokomo Arnold | 2:44 | |
| Prayer of a Miner's Child | Dock Boggs | 3:21 | |
| You Get Down by the Pool Hall Clickety Clack - Sister Song | Michael Hurley | 3:34 | |
| K. C. Railroad Blues | Andrew & Jim Baxter | 3:30 | |
| Catfish Blues | Skip James | 4:37 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |