| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Ninety-Nine Year Blues | Julius Daniels | 3:06 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Hell Hound On My Trail | Robert Johnson | 2:37 | |
| Hiram Hubbard | Jean Ritchie and Doc Watson | 3:50 | |
| Mississippi Bottom Blues | Kid Bailey | 2:49 | |
| I Get' the Blues | Bo Carter | 2:55 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Cumberland Gap | Dock Boggs | 2:29 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Evil Devil Woman Blues | Memphis Minnie & Kansas Joe | 3:14 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Southbound | The Doc Watson Family | 2:40 | |
| When First Into this Country | The New Lost City Ramblers | 2:48 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Harvey Logan | Dock Boggs | 3:33 | |
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| Go Ahead, Buddy | Casey Bill Weldon, Kokomo Arnold | 2:44 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Old Rub Alcohol Blues | Dock Boggs | 3:10 | |
| I Will Turn Your Money Green (Alternate Take) | Furry Lewis | 3:11 | |
| John Hardy | Mike Seeger | 2:44 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| The Fox and the Hounds | Henry Thomas | 2:36 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| When I Die | The Doc Watson Family | 2:17 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Sweet Sweet Thing | The Delmore Brothers | 2:52 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Cuba | Dock Boggs | 1:29 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| Goodbye booze | Charlie Poole & The North California Ramblers, Charlie Poole | 3:20 | |
| When Death Come Creeping in Your Room (Run, Sinner, Run) - Take 1; Remastered Version | Mance Lipscomb | 2:57 | |
| Let's Disagree | Leroy Carr | 2:56 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Goodbye Blues | Blind Willie McTell | 1:39 | |
| Crow Jane | Skip James | 2:58 | |
| Roberta | Furry Lewis | 2:34 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| Little Sadie | Norman Blake | 1:50 | |
| Easy Rider Blues | Blind Lemon Jefferson | 2:54 | |
