| Sunshine Special | Blind Lemon Jefferson | 2:46 | |
| Black Angel Blues | Robert Nighthawk | 3:04 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Rollin' Stone, Pt. 1 | Robert Wilkins | 2:53 | |
| Cold Blooded Woman | Memphis Slim | 3:39 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Police Station Blues | Peetie Wheatstraw | 3:05 | |
| Mother's Children Have a Hard Time | Blind Willie Johnson | 3:21 | |
| Jump Steady Daddy | Lucille Bogan | 2:47 | |
| Memphis Blues | W.C. Handy, Victor Military Band | 3:02 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Dark Road Blues | Willie Lofton | 3:03 | |
| Devil Sent the Rain Blues | Charley Patton | 3:05 | |
| Dying Gambler's Blues | Bessie Smith | 2:56 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| My Black Mama, Pt. 1 | Son House | 3:05 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Alabama March | J.B. Lenoir | 2:53 | |
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| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Beefsteak Blues | James "Son" Thomas | 2:55 | |
| County Jail Blues | Big Maceo | 2:55 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Pony Blues | Son House | 5:22 | |
| Let Me Play With Your Noodle - Remastered | Tampa Red | 2:36 | |
| The Rain Don't Fall on Me | Blind Willie Johnson | 3:18 | |
| Floating Bridge | Sleepy John Estes | 3:12 | |
| Rockin Chair Blues | Robert Petway | 3:01 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| When Can I Change My Clothes? | Bukka White | 2:59 | |
| Ramblin' Blues | Charlie Poole | 3:07 | |
| Hot Dogs | Blind Lemon Jefferson | 2:58 | |
| The Twelves (The Dirty Dozens) | Casey Bill Weldon, Kokomo Arnold | 3:11 | |
| Someday Baby Blues | Sleepy John Estes | 2:59 | |
| Anna Lou Blues | Tampa Red | 2:54 | |
| It's Nobody's Fault but Mine | Blind Willie Johnson | 3:08 | |
| High And Lonesome | Jimmy Reed | 2:47 | |
| Don't Dog Your Woman | J.B. Lenoir | 3:13 | |
| Rising High Water Blues | Blind Lemon Jefferson | 2:28 | |
| Act Like You Love Me | Jimmy Rogers | 3:53 | |
| Catfish Blues | Skip James | 4:37 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Come Back Baby | Doctor Ross | 2:47 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| My Baby Left Me This Morning | Pink Anderson | 3:38 | |