| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Feelin' Good | J.B. Lenoir | 3:01 | |
| Two Bugs and a Roach | Earl Hooker | 4:19 | |
| Levee Camp Blues | Son House | 3:45 | |
| Yonder Comes The Blues | Stefan Grossman | 5:23 | |
| It's Nobody's Fault but Mine | Blind Willie Johnson | 3:08 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| Cocaine Blues | Reverend Gary Davis | 2:40 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| Bukka Jitterburg Swing | Bukka White | 2:35 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| Crossroads | David "Honeyboy" Edwards | 3:26 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Walking Blues | Son House, Willie Brown, Fiddlin’ Joe Martin, Leroy Williams | 6:35 | |
| That's All Right | Arthur "Big Boy" Crudup | 2:53 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Ain't Seen My Baby | Cephas & Wiggins | 3:22 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Catfish Blues (Alternate Take) | Robert Petway | 2:54 | |
| Alabama March | J.B. Lenoir | 2:53 | |
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| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| Children of Zion | Rev. Gary Davis | 7:09 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| T-Bone Blues | T-Bone Walker | 3:48 | |
| It Hurts Me Too | Elmore James | 3:12 | |
| Captain, Captain - Version; Take 2; Remastered | Mance Lipscomb | 2:34 | |
| Slow Down | J.B. Lenoir | 1:34 | |
| What's That Smells Like Fish | Blind Boy Fuller | 2:46 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| I Heard The Angels Sing | Rev. Gary Davis | 4:18 | |
| Letter from Bluewater Man | Christone "Kingfish" Ingram | 2:24 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| It Hurts Me Too | Tampa Red | 2:33 | |
| Falling Down Blues | Furry Lewis | 2:48 | |
| Washington D.C. Hospital Center Blues | Skip James | 4:11 | |
| Old Blue | Furry Lewis | 3:00 | |
| I'm A King Bee | Slim Harpo | 3:02 | |
| My Journey To The Sky | Sister Rosetta Tharpe, Marie Knight, Sam Price Trio | 3:10 | |
| Catfish Blues | Skip James | 4:37 | |
| Checkin' Up On My Baby - Single Version | Sonny Boy Williamson II | 1:57 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Blue And Lonesome - Single Version | Little Walter | 2:54 | |
| Death Letter | Son House | 5:51 | |