| New Orleans (The Rising Sun Blues) | Lead Belly | 3:16 | |
| 34 Blues | Charley Patton | 2:57 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| Charley Bostock's Blues | C.W. Stoneking | 3:34 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Dope Head Blues | Victoria Spivey | 3:15 | |
| One Dime Blues | Blind Lemon Jefferson | 2:48 | |
| Mississippi Boweavil Blues | Charley Patton | 3:11 | |
| My Mama Don't Allow Me | Arthur "Big Boy" Crudup | 3:10 | |
| The House Carpenter - 2020 Remaster | Clarence Ashley | 3:18 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| It Hurts Me Too | Elmore James | 3:20 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| St. James Infirmary | Josh White | 3:42 | |
| One Dime Blues | Etta Baker | 3:43 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Devil Sent the Rain Blues | Charley Patton | 3:05 | |
| Last Kind Word Blues | Geeshie Wiley | 3:04 | |
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| John Henry | Henry Thomas | 2:43 | |
| Levee Camp Blues | Son House | 3:45 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Rock Me Mama | Arthur "Big Boy" Crudup | 2:29 | |
| I Am The Light | Rev. Gary Davis | 3:02 | |
| Gambling Bar Room Blues | Jimmie Rodgers | 3:23 | |
| Alabama Bound - Version; Take 2; Remastered | Mance Lipscomb | 2:36 | |
| The Coo Coo Bird | Clarence Ashley | 2:56 | |
| Sweeter as the Years Roll By | Blind Willie Johnson | 2:46 | |
| Chasin' Rainbows | R. Crumb And His Cheap Suit Serenaders | 3:17 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Eighteen Hammers | Johnny Lee Moore | 2:37 | |
| High Sheriff Blues | Charley Patton | 3:10 | |
| John the Revelator | Son House | 2:29 | |
| Keep Your Lamp Trimmed And Burning | Corey Harris | 2:54 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| I Got Mine | Frank Stokes | 3:06 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Ira Hayes (Ballad of Ira Hayes) | Peter La Farge | 3:35 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Homesick and Lonesome Blues | Blind Boy Fuller | 3:09 | |
| Standing In My Doorway Crying | Jessie Mae Hemphill | 4:40 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Don't Take Everybody To Be Your Friend | Sister Rosetta Tharpe, Sam Price Trio | 2:25 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Baby Please Don't Go | Pink Anderson | 2:45 | |