| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Gather Ye Rosebuds While Ye May | Bayard Rustin | 2:22 | |
| Levee Camp Moan | Son House | 9:27 | |
| Saturday Blues | Ishman Bracey | 3:29 | |
| She Left Me a Mule to Ride | Big Joe Williams | 2:35 | |
| My Trouble Is Hard | Jubalaires | 2:05 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| All Night Long Blues | Louise Johnson, Willie Brown | 3:13 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Leatherwing Bat | Peggy Seeger | 2:11 | |
| Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3:18 | |
| Down South Blues | Dock Boggs | 2:11 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Down By The Riverside | Sonny Terry and Brownie McGhee | 3:16 | |
| You Missed a Good Man | Tampa Red | 3:34 | |
| Clouds for Me | Shevon Bennett | 1:43 | |
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| Give Me My Flowers (While I yet Live) | Gee's Bend Quilters | 3:09 | |
| Jesus Is My Friend | Washington Phillips | 2:53 | |
| New Jerusalem | Sister Gertrude Morgan | 2:23 | |
| Stack O' Lee Blues | Ma Rainey | 2:57 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:05 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| Born Dead | J.B. Lenoir | 3:47 | |
| The Atomic Telephone | The Spirit Of Memphis Quartet | 2:39 | |
| It's Nobody's Fault but Mine | Blind Willie Johnson | 3:08 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Dry Southern Blues | Blind Lemon Jefferson | 2:53 | |
| Denomination Blues, Pt. 2 | Washington Phillips | 2:38 | |
| Jesus Is Real to Me | Mary Lee | 3:14 | |
| Dark Was the Night | Blind Willie Johnson | 3:24 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Take Your Burden to the Lord and Leave It There | Washington Phillips | 3:15 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Falling Down Blues | Furry Lewis | 2:48 | |
| Diddie Wa Diddie | Blind Blake | 3:00 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| I Saw Her as I Came and Went | Bayard Rustin | 2:03 | |
| The Sun Will Never Go Down | Sweet Honey In The Rock | 2:23 | |
| Jesus Hits Like The Atom Bomb | The Pilgrim Travelers | 2:25 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
| Old Blue | Furry Lewis | 3:00 | |