| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Betty Baker | Chance McCoy & The Appalachian String Band | 2:50 | |
| Sittin' by the Road | Blaze Foley | 2:18 | |
| Brown's Ferry Blues | The New Lost City Ramblers | 2:49 | |
| Poor Boy | Harry McClintock | 2:14 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Salt Creek | Clarence White | 2:39 | |
| Last Chance | Hobart Smith | 1:30 | |
| Suwannee River | Mary Z. Cox | 0:57 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Rambling Gambler | Alan Lomax | 3:08 | |
| Sweet Sweet Thing | The Delmore Brothers | 2:52 | |
| Johnny Booker | The New Lost City Ramblers, Cousin Emmy | 1:32 | |
| Jane Jane | Peggy Seeger | 1:32 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Lonesome Yodel Blues | The Delmore Brothers | 2:37 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
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| Comin' Round the Mountain | Uncle Dave Macon, Sam McGee | 3:07 | |
| Cumberland Gap | Frank Fairfield | 3:33 | |
| Jimmy Johnson | Chance McCoy & The Appalachian String Band | 3:13 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Jennie Jenkins | The New Lost City Ramblers | 2:34 | |
| La Le Too Dum | Barbara Dane | 3:18 | |
| Shortening Bread | Cousin Emmy, The New Lost City Ramblers | 1:09 | |
| Billy in the Low Ground | Clarence White | 1:37 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Shenandoah | Paul Clayton | 2:27 | |
| The Golden Vanity | Jody Stecher | 5:28 | |
| Ozark Bill | Jimmy Driftwood | 2:46 | |
| Reuben Train | Frank Proffitt | 2:57 | |
| The Fox and the Hounds | Henry Thomas | 2:36 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Ramblin' | Barbara Dane | 2:57 | |
| The Hills Of Isle Au Haut | Jody Stecher | 3:33 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Queen of the Rails | Utah Phillips | 2:35 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Barnyard Banjo Pickin' | Stringbean | 2:18 | |
| Roll On Buddy | The Kentucky Colonels | 2:18 | |
| Lorene | The Louvin Brothers | 2:03 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| I'm Bound To Ride | Fiddlin' Arthur Smith, His Dixieliners | 2:19 | |
| Old Home Place | J.D. Crowe & The New South | 2:48 | |
