| County Farm Blues | Son House | 2:12 | |
| Do the Romp | Junior Kimbrough | 3:57 | |
| You Gonna Take Sick and Die | Boyd Rivers | 3:41 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Drop Down Mama | Sleepy John Estes | 3:11 | |
| Blues For The Lowland | Sonny Terry and Brownie McGhee | 4:46 | |
| Mean Ol' Frisco | Arthur "Big Boy" Crudup | 2:37 | |
| Beefsteak Blues | James "Son" Thomas | 2:55 | |
| Sittin' On Top of the World | Sam Chatmon | 3:00 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Things 'Bout Comin' My Way | Tampa Red | 3:13 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Alabama March | J.B. Lenoir | 2:53 | |
| John Hardy Was a Desperate Little Man | The Carter Family | 2:55 | |
| Stones In My Passway | Robert Johnson | 2:28 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Crow Jane | Etta Baker, Cora Phillips | 2:21 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Papa's on the House Top | Leroy Carr | 2:55 | |
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| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Going Down the Road Feeling Bad | Etta Baker | 2:37 | |
| That's No Way to Get Along (Alternate Take) | Robert Wilkins | 2:53 | |
| Rocks and Gravel Makes a Solid Road - Take 6; Remastered Version | Mance Lipscomb | 4:01 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| John Henry | Furry Lewis | 4:51 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | |
| Catfish Blues (Alternate Take) | Robert Petway | 2:54 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| I Got Mine | Frank Stokes | 3:06 | |
| Tears Don't Fall No More | Lonnie Johnson | 3:11 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| That's All Right, Pt. 1 | John Henry Barbee | 3:40 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| My Baby Left Me - Remastered 2003 | Arthur "Big Boy" Crudup | 2:21 | |
| Slow Drag (Cincinnati Flow Rag) - Instrumental | Rev. Gary Davis | 2:24 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Moaning Blues | Lonnie Johnson | 4:03 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |
| Haunted House | Lonnie Johnson, Elmer Snowden | 5:00 | |
| Graveyard Blues | Robert Pete Williams | 2:46 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Change My Luck Blues | Blind Lemon Jefferson | 2:24 | |
| Jelly Whippin' Blues | Tampa Red | 3:51 | |
| Black Cat Bone | Jessie Mae Hemphill | 4:57 | |
| Luedella | Jimmy Rogers | 2:54 | |