| The Prodigal Son | Robert Wilkins | 10:00 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Garter Snake Blues | Victoria Spivey | 3:17 | |
| 32-20 Blues | Robert Johnson | 2:47 | |
| You Gonna Take Sick and Die | Boyd Rivers | 3:41 | |
| A Lazy Farmer Boy | Buster Carter & Preston Young | 3:01 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| New Jerusalem | Sister Gertrude Morgan | 2:23 | |
| Black Mountain Blues | Bessie Smith | 3:06 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| W.P.A. Blues | Casey Bill Weldon | 3:17 | |
| Pin in Your Cushion | Bo Carter | 3:06 | |
| Tom Rushen Blues | Charley Patton | 3:07 | |
| Jail-House Blues | Bessie Smith | 3:13 | |
| Tight in Chicago | Mozelle Alderson | 2:59 | |
| Dark Holler Blues | Clarence Ashley | 2:58 | |
| Terraplane Blues | Robert Johnson | 2:58 | |
| Cocaine | Dick Justice | 3:12 | |
| See See Rider | Wee Bea Booze | 2:36 | |
| Future Blues | Willie Brown | 3:01 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
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| Romance In The Dark | Lil Green | 3:23 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Mean Old Bedbug Blues | Bessie Smith | 3:11 | |
| The Coo Coo Bird | Clarence Ashley | 2:56 | |
| Your Love Is Where It Ought to Be | Big Mama Thornton | 3:49 | |
| Sugar Mama Blues | Sonny Boy Williamson I | 2:58 | |
| When You Got a Good Friend - Take 2 | Robert Johnson | 2:50 | |
| Pig Meat Is What I Crave | Bo Carter | 2:51 | |
| See See Rider Blues | Ma Rainey | 3:15 | |
| Evening Sun | Johnny Shines | 2:32 | |
| Moan, You Moaners | Bessie Smith | 3:11 | |
| Mean Old Bedbug Blues | Furry Lewis | 2:45 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| The Girl I Love, She Got Long Curly Hair | Sleepy John Estes | 2:56 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Worried Life Blues | Big Maceo | 2:54 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Preachin' Blues (Up Jumped the Devil) | Robert Johnson | 2:48 | |
| Take A Walk With Me (Take2) | Robert Lockwood, Jr., JR. | 3:00 | |
| Cocaine Blues | Luke Jordan | 3:18 | |
| Stack O' Lee Blues | Ma Rainey | 2:57 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| Tallahatchie River Blues | Mattie Delaney | 2:49 | |
| Trouble, I've Had It All My Days | Mississippi John Hurt | 3:04 | |
| Tallahatchie River Blues - Single Version | Mattie Delaney | 2:49 | |
| Great Change Since I Been Born | Rev. Gary Davis | 4:03 | |
| Goin' Away Baby - Single Version | Jimmy Rogers | 2:46 | |
| Levee Break Blues | Big Joe Williams | 1:53 | |