| Beale Street Sugar | Sleepy John Estes, The Tennessee Jug Busters, Mike Bloomfield | 3:53 | |
| I Wish You Would | Billy Boy Arnold | 3:39 | |
| Sugar Diabetes Blues | Big Joe Williams | 2:46 | |
| Get Your Yas Yas Out | Blind Boy Fuller | 2:28 | |
| Roll and Tumble Blues - 1929 Version | Hambone Willie Newbern | 3:04 | |
| Mean Ol' Frisco | Arthur "Big Boy" Crudup | 2:37 | |
| That Ain't Your Business | Slim Harpo | 2:12 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Rooster Blues | Lightnin' Slim | 2:32 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Friendless Blues | Lonnie Johnson | 3:02 | |
| Evening Sun | Johnny Shines | 2:32 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Wild About You, Baby | Hound Dog Taylor & The HouseRockers | 3:39 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Mean Old Twister | Kokomo Arnold | 2:59 | |
| My Black Mama, Pt. 1 | Son House | 3:05 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Cotton Crop Blues | James Cotton | 3:01 | |
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| Someday Baby Blues | Sleepy John Estes | 2:59 | |
| Talk to Your Daughter | J.B. Lenoir | 2:45 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| When the Levee Breaks | Memphis Minnie | 3:11 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| So Glad I'm Livin' | Sleepy John Estes, The Tennessee Jug Busters | 2:37 | |
| Ride 'Em on Down | Eddie Taylor | 2:57 | |
| Dig Myself a Hole | Arthur "Big Boy" Crudup | 2:23 | |
| Baby Don't You Lay It on Me | Mance Lipscomb | 2:24 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Am I Right Or Wrong? | Son House | 1:45 | |
| Feelin' Good | J.B. Lenoir | 3:01 | |
| I Found a New Love | Magic Sam, Earnest Johnson, Mighty Joe Young, Odie Payne, Jr., Stockholm Slim | 4:08 | |
| Oh Death | Charley Patton | 2:52 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Death Letter Blues | Son House | 4:19 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Long Road to Travel | Lonnie Johnson | 2:22 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Sittin' Here Wondering | Slim Harpo | 3:19 | |
| Blues All Day Long (Blues Leave Me Alone) - Single Version | Jimmy Rogers | 3:05 | |
| Shake 'Em On Down | Furry Lewis | 2:50 | |
| Coffee Blues | Mississippi John Hurt | 3:46 | |
| Midnight Hour | Clarence "Gatemouth" Brown | 2:46 | |