| Willow Garden | Roscoe Holcomb | 3:27 | |
| Aunt Caroline Dyer Blues | Memphis Jug Band | 3:06 | |
| Nobody's Business | Riley Puckett | 2:40 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Train on the Island | Hazel Dickens, Alice Gerrard | 1:44 | |
| Poor Ellen Smith | The Country Gentlemen | 2:16 | |
| Hill Billie Blues - Remastered | Uncle Dave Macon | 2:57 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Detour | Spade Cooley | 2:38 | |
| Walkin' in My Sleep | Hazel Dickens, Alice Gerrard | 2:10 | |
| Leaving dear old Ireland | Charlie Poole & The North California Ramblers, Charlie Poole | 3:04 | |
| John Hardy | Mike Seeger | 2:44 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Pussy Cat Blues | Bo Carter | 2:54 | |
| The Highwayman | Charlie Poole | 3:20 | |
| Long Lonesome Road | Peggy Seeger | 2:17 | |
| James Alley Blues | Richard "Rabbit" Brown | 3:09 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
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| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Last Chance | Hobart Smith | 1:30 | |
| Knife Guitar | Roscoe Holcomb | 1:17 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Trail Of Sorrow | Reno & Smiley | 2:19 | |
| Bill Bailey | Big Bill Broonzy | 1:57 | |
| We Have Moonshine In The West Virginia Hills | Earl Shirkey & Roy Harper | 3:01 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| John Henry | Big Bill Broonzy | 4:44 | |
| Talking Banjo | Buzz Busby | 1:39 | |
| Cocaine | Dick Justice | 3:12 | |
| Bowling Green | The New Lost City Ramblers, Cousin Emmy | 1:50 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Cigareetes, Whusky and Wild, Wild Women | Sons of the Pioneers | 2:54 | |
| Jesse James | Harry McClintock | 3:28 | |
| Won't You Come and Sing for Me? - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:45 | |
| Bugger Burns | Spider John Koerner, Dave Ray, Tony Glover | 1:40 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Rattlesnake | Alan Lomax | 1:34 | |
| Mississippi County Farm Blues | Son House | 2:50 | |
| Milwaukee Here I Come | Jimmy Martin | 2:37 | |
| False Hearted Lover's Blues | Dock Boggs | 3:27 | |
| My Wife, She Has Gone and Left Me (with Kelly Harrell & The Virginia String Band) | Charlie Poole, Kelly Harrell & The Virginia String Band | 3:16 | |