| La Danseuse | Delma Lachney | 2:54 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Hangman | Spider John Koerner, Dave Ray, Tony Glover | 2:32 | |
| You Can't Tell the Difference After Dark | Alberta Hunter | 2:59 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Let Me Play With Your Noodle - Remastered | Tampa Red | 2:36 | |
| Last Kind Word Blues (Alternate Take 2) | Geeshie Wiley | 2:58 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Night Time Is the Right Time | Mance Lipscomb | 4:00 | |
| Fourth Street Mess Around | Memphis Jug Band | 3:16 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Meningitis Blues | Memphis Minnie, Memphis Jug Band | 3:16 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| See See Rider Blues | Ma Rainey | 3:15 | |
| Hoodoo Lady | Memphis Minnie | 3:02 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
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| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Fallin' Down Blues | Robert Wilkins | 2:38 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Frankie & Johnny | Champion Jack Dupree | 3:23 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| Cotton Crop Blues | James Cotton | 3:01 | |
| W.P.A. Blues | Casey Bill Weldon | 3:17 | |
| Pistol slappin' blues | Blind Boy Fuller | 2:39 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Can you Blame The Colored Man | Gus Cannon | 2:46 | |
| You May Leave But This Will Bring You Back | Memphis Jug Band | 3:03 | |
| Strut That Thing | Cripple Clarence Lofton | 2:59 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| I Got Mine | Frank Stokes | 3:06 | |
| Black Snake Moan (Alternate Take) | Blind Lemon Jefferson | 3:03 | |
| High And Lonesome | Jimmy Reed | 2:47 | |
| John Henry | Furry Lewis | 5:49 | |
| Washington D.C. Hospital Center Blues | Skip James | 4:11 | |
| Mary Anna Cut Off | Memphis Jug Band | 3:11 | |
| By The Spoonful | J.D. Short | 3:49 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| Shake 'Em On Down | Furry Lewis | 2:50 | |
| Maple Leaf Rag - Instrumental | Rev. Gary Davis | 2:55 | |