| I Be so Glad When the Sun Goes Down | Ed Lewis | 2:36 | |
| Paul And Silas In Jail | Washington Phillips | 2:41 | |
| Woke up This Morning | Fannie Lou Hamer | 2:31 | |
| John Henry | Henry Thomas | 2:43 | |
| Turkle Dove | Bessie Jones, Georgia Sea Island Singers | 1:43 | |
| Keep My Skillet Good and Greasy | Sid Hemphill | 3:37 | |
| The Atomic Telephone | The Spirit Of Memphis Quartet | 2:39 | |
| Goodbye My Riley O - Live | Georgia Sea Island Singers, John Davis | 2:41 | |
| Shoo Turkey | Bessie Jones | 1:04 | |
| If I Had My Way | Rev. Gary Davis | 4:46 | |
| Rattler | Mose (Clear Rock) Platt, Alan Lomax | 1:03 | |
| Columbiana | Various Artists, Alan Lomax | 2:34 | |
| All Night Long - Remastered | Miles Pratcher, Bob Pratcher, Alan Lomax | 3:04 | |
| I Shall Not Be Moved | Shirley Ann Lee | 2:52 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| I Had A Good Father And Mother | Washington Phillips | 3:00 | |
| Let Your Light Shine on Me | Blind Willie Johnson | 3:08 | |
| I'll Meet You On that Other Shore | St. James Primitive Baptist Church congregation, Alan Lomax | 5:44 | |
| I'll Be So Glad When the Sun Goes Down - Remastered | Ed Young, Unidentified prisoners | 5:01 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
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| Way Down Yonder in the Brickyard | Bessie Jones | 1:18 | |
| Once There Was No Sun - Live | Georgia Sea Island Singers, Bessie Jones | 2:40 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Couldn't Hear Nobody Pray | Bernice Johnson Reagon | 1:49 | |
| Woke Up This Morning With My Mind On Jesus - Remastered | Mississippi Fred McDowell | 3:18 | |
| Move on Up A Little Higher | The Dixie Hummingbirds | 2:45 | |
| Booze and Blues | Ma Rainey | 3:11 | |
| Chevrolet | Lonnie Young, Ed Young | 4:04 | |
| Steel Laying Holler | Rochelle Harris, Alan Lomax | 2:24 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Troubled, Lord I'm Troubled | Bill Landford | 2:53 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Shallow Water | Donald Harrison, Dr. John | 3:03 | |
| The Old Ship of Zion - Previously Unreleased | Ishman Williams & the William Singers | 4:32 | |
| Jim and John | Ed Young, Lonnie Young, G.D. Young | 3:08 | |
| Set Down, Servant | Adie Corbin and Ed Frierson, Alan Lomax | 2:16 | |
| Trust in the Lord | Johnnie Frierson | 4:10 | |
| Ever Since | Pastor T.L. Barrett and the Youth for Christ Choir | 4:32 | |
| Joe Lee's Rock | Boy Blue | 3:36 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
| I'm Going Home - Remastered | Ervin Webb, Unidentified prisoners | 3:25 | |
| Motherless Child | The Harmonizing Four | 3:09 | |
| Interview #2 - Plantation Recording | Muddy Waters, Alan Lomax, John Work | 1:50 | |
| Nobody Knows The Trouble I See | The Dixie Hummingbirds | 3:01 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Lord, Don't Forget About Me | Dorothy Love Coates, The Original Gospel Harmonettes | 2:14 | |
| Another Man Done Gone | Vera Hall | 1:24 | |