| Pretty Polly Oliver - Remastered | Ollie Gilbert, Alan Lomax | 2:33 | |
| Baby Please Don't Go | Big Joe Williams | 2:44 | |
| Old Gospel Ship - Remastered | Ruby Vass, Alan Lomax | 2:56 | |
| Morning Prayer | Various Artists, Alan Lomax | 2:33 | |
| Mardi Gras in New Orleans (Reprise No. 1) | Professor Longhair | 2:53 | |
| Couldn't Hear Nobody Pray | Bernice Johnson Reagon | 1:49 | |
| Shout Lula | Emma Hammond, Alan Lomax | 1:00 | |
| The House Carpenter - 2020 Remaster | Clarence Ashley | 3:18 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Country Blues | Dock Boggs | 3:55 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Columbiana | Various Artists, Alan Lomax | 2:34 | |
| I Am The Light | Rev. Gary Davis | 3:02 | |
| The Girl I Left Behind - Remastered | Spencer Moore, Roy Everett Blevins, Alan Lomax | 2:52 | |
| Wild Women Don't Have the Blues | Ida Cox | 2:27 | |
| Swing Low, Sweet Chariot | The Fisk Jubilee Singers | 3:26 | |
| Sally Anne | Alan Lomax, Norman Edmonds & the Old-Timers | 2:26 | |
| If I Had My Way | Rev. Gary Davis | 4:46 | |
| My Trouble Is Hard | Jubalaires | 2:05 | |
| Introduction by Alan Lomax / Travelin' Shoes - Live | Georgia Sea Island Singers, Bessie Jones | 2:29 | |
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| Ida Reed - Remastered | Ed Young, Lonnie Young Sr., G. D. Young, Alan Lomax | 2:17 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Turkle Dove | Bessie Jones, Georgia Sea Island Singers, Alan Lomax | 1:43 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Jesus On the Mainline - Remastered | James Shorter, Viola James, Independence Church congregation, Alan Lomax | 3:40 | |
| Sing Anything - Remastered | Neal Morris, Alan Lomax | 2:12 | |
| Señorita Panchita | Neville Marcano (The Growling Tiger), Alan Lomax | 3:07 | |
| Go Tell It on the Mountain | Fannie Lou Hammer | 3:05 | |
| O Death | Bessie Jones, Alan Lomax | 3:48 | |
| This Little Light of Mine | Fannie Lou Hamer | 3:03 | |
| I Had A Good Father And Mother | Washington Phillips | 3:00 | |
| Kenny Wagner | Bob Carpenter | 2:34 | |
| Blow, Gabriel | The McIntosh County Shouters | 3:37 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| John Henry | W.D. Stewart (Bama), Benny Will Richardson (22), Unidentified prisoners, Alan Lomax | 3:49 | |
| Rolled and Tumbled - Remastered | Rosalie Hill, Alan Lomax | 2:56 | |
| Trust in the Lord | Johnnie Frierson | 4:10 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| Interview: Demonstration of Concert Guitar Tuning | Son House, Alan Lomax | 1:29 | |
| Woke up This Morning | Fannie Lou Hamer | 2:31 | |
| Rambling Gambler | Alan Lomax | 3:10 | |
| Sheep, Sheep | Georgia Sea Island Singers | 2:21 | |
| Trouble in Mind | Roscoe Holcomb | 2:18 | |
| Train 111 - Remastered | J.E. Mainer's Mountaineers, Alan Lomax | 2:59 | |
| Ezekiel Saw The Wheel | The Dixie Hummingbirds | 1:33 | |
| Lord, Don't Forget About Me | Dorothy Love Coates, The Original Gospel Harmonettes | 2:14 | |
| Another Man Done Gone | Vera Hall | 1:24 | |
| Daniel In The Lion's Den | Bessie Jones | 2:56 | |
