| The Old Tar River | John Davis and the Georgia Sea Island Singers, Alan Lomax | 2:31 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| I Had A Good Father And Mother | Washington Phillips | 3:00 | |
| Introduction by Alan Lomax / Travelin' Shoes - Live | Georgia Sea Island Singers, Bessie Jones | 2:29 | |
| Wade in the Water | The Fisk Jubilee Singers | 2:24 | |
| Tribulations - Remastered | E.C. Ball | 2:50 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Once There Was No Sun - Live | Georgia Sea Island Singers, Bessie Jones | 2:40 | |
| Hog Hunt | Sid Hemphill | 4:40 | |
| Señorita Panchita | Neville Marcano (The Growling Tiger), Alan Lomax | 3:07 | |
| John the Revelator | Son House | 2:29 | |
| O Death | Bessie Jones | 3:46 | |
| High Fever Blues | Bukka White | 2:50 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Lazarus - Remastered | Henry Morrison, Alan Lomax | 1:42 | |
| Grinnin' In Your Face | Son House | 1:56 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Huntington | Various Artists, Alan Lomax | 2:37 | |
| Diggin' My Potatoes | Washboard Sam | 2:58 | |
| Big Chief - Complete Version | Professor Longhair | 4:30 | |
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| Rope Stretching Blues | Blind Blake | 3:02 | |
| Hallelujah + Amazing Grace | United Sacred Harp Musical Association | 1:59 | |
| Oh Mary, Don't You Weep (feat. Kim Harris & Reggie Harris) | Bernice Johnson Reagon, Reggie Harris, Kim Harris | 3:47 | |
| Father Blues | Olu Dara | 3:31 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Chilly Winds - Remastered | Wade Ward | 1:24 | |
| Jesus On the Mainline - Remastered | James Shorter, Viola James | 3:40 | |
| Jennie Jenkins | Estil C. Ball & Orna Ball | 2:42 | |
| See Aunt Dinah | Bessie Jones, Georgia Sea Island Singers, Alan Lomax | 1:34 | |
| I'll Fly Away | Marion Williams | 2:49 | |
| Reg'lar, Reg'lar, Rolling Under - Remastered | Bessie Jones | 2:23 | |
| John Henry | Henry Thomas | 2:43 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Dead and Gone | Bessie Jones | 2:18 | |
| Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3:18 | |
| Sherburne (186) - Remastered | United Sacred Harp Convention | 1:50 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Goodbye Honey, You Call That Gone | Lucius Smith, Alan Lomax | 2:13 | |
| That da da Strain | Mamie Smith | 2:52 | |
| I Shall Not Be Moved | Shirley Ann Lee | 2:52 | |
| Which Side are You On? | The Freedom Singers | 3:47 | |
| Early In The Morning | Alan Lomax | 5:49 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |
| Kindlin' Wood | Bessie Jones | 7:47 | |
| Louisiana | Percy Mayfield | 2:02 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Mary Don't You Weep | The Harmonizing Four | 2:29 | |
| Lord, Don't Forget About Me | Dorothy Love Coates, The Original Gospel Harmonettes | 2:14 | |