| Marie La Veau | Papa Celestin | 6:36 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Ain't Gonna Let Nobody Turn Me 'Round | The Freedom Singers | 2:34 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Black Betty | James "Iron-Head" Baker, R.D. Allen, Will Crosby | 1:28 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| Take Your Burden to the Lord and Leave It There | Washington Phillips | 3:15 | |
| My Handy Man Ain't Handy No More | Alberta Hunter | 3:46 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| None of My Jelly Roll | Sweet Emma Barrett | 3:45 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Talkin' Lion Blues | C.W. Stoneking | 4:33 | |
| Keep My Skillet Good and Greasy | Sid Hemphill | 3:37 | |
| The Girl I Left Behind - Remastered | Spencer Moore, Roy Everett Blevins, Alan Lomax | 2:52 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| Ain't Gonna Let Nobody Turn You Round | The Freedom Singers | 2:31 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| James Alley Blues | Richard "Rabbit" Brown | 3:09 | |
| I Know His Blood Can Make Me Whole | Blind Willie Johnson | 3:02 | |
| Drinking That Wine | Bessie Jones, Alan Lomax | 1:43 | |
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| Black Woman (Wild Ox Moan) - Previously Unreleased Version | Vera Hall | 2:08 | |
| Couldn't Hear Nobody Pray | Bernice Johnson Reagon | 1:49 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| I'll Keep Sittin' On It | Georgia White | 2:58 | |
| La Partenza (The Parting) | Trallaleri of Genoa, Alan Lomax | 2:45 | |
| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| Logan | Various Artists, Alan Lomax | 2:08 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Why Must I Wear This Shroud? - Remastered | George Spangler & Thornton Old Regular Baptist Church congregation, Alan Lomax | 5:16 | |
| Looking for a Sweetie | Lonnie Johnson | 3:48 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| Heaven Is Mine | Unidentified woman, Pentecostal Temple congregation, Alan Lomax | 3:37 | |
| Work With Me Annie | Hank Ballard | 3:05 | |
| I'll Meet You On that Other Shore | St. James Primitive Baptist Church congregation, Alan Lomax | 5:44 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Josephine - Live | Georgia Sea Island Singers, Bessie Jones | 1:14 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Good Luck Charm | C.W. Stoneking | 2:45 | |
| John Henry | Furry Lewis | 4:51 | |
| John Henry | Unidentified Mississippi State Penitentiary (Oakley) prisoners, Alan Lomax | 2:39 | |
| Chasin' Rainbows | R. Crumb And His Cheap Suit Serenaders | 3:17 | |
| Oh Death | Dock Boggs | 3:22 | |
| Lu Sand'Andonie | Alan Lomax | 1:38 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Daniel In The Lion's Den | Bessie Jones | 2:56 | |
| Jealous Hearted Blues | Ma Rainey | 3:10 | |
| Motherless Child | The Harmonizing Four | 3:09 | |
| Boll Weevil | Alan Lomax | 2:54 | |