| My Mama Don't Allow Me | Arthur "Big Boy" Crudup | 3:10 | |
| In His Foosteps | God's Deliverance Singers, Henry Thomas | 5:31 | |
| Mule Skinner Blues | Bill Monroe & His Blue Grass Boys | 2:45 | |
| When the Sun Goes Down | Leroy Carr | 2:59 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| Country Blues | Dock Boggs | 3:55 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Gambling Bar Room Blues | Jimmie Rodgers | 3:23 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| The Soul of a Man | Blind Willie Johnson | 3:12 | |
| Blues Everywhere | Memphis Minnie | 2:49 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
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| I'm Troubled | The Doc Watson Family | 2:42 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| Blue Yodel No. 2 (My Lovin' Gal Lucille) | Jimmie Rodgers | 3:17 | |
| Lovin' Babe | Henry Thomas | 3:05 | |
| Pussy Cat Blues | Bo Carter | 2:54 | |
| Stealin' Stealin' | Memphis Jug Band | 2:56 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Blues Stay Away from Me | The Delmore Brothers | 2:49 | |
| Roll and Tumble Blues - 1929 Version | Hambone Willie Newbern | 3:04 | |
| Swing Low Sweet Chariot | Sister O.M. Terrell | 2:36 | |
| Levee Camp Blues | Son House | 3:45 | |
| Texas Worried Blues | Henry Thomas | 3:18 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Skin Game Blues | Peg Leg Howell | 3:05 | |
| Sic 'Em Dogs On | Bukka White | 2:27 | |
| My Pencil Won't Write No More | Bo Carter | 2:57 | |
| Darling Cory | Roscoe Holcomb | 3:42 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Pig Meat Is What I Crave | Bo Carter | 2:51 | |
| John Henry | Henry Thomas | 2:46 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| Fly Around My Pretty Little Miss | Ola Belle Reed | 2:00 | |
| Standing At The Crossroads | Johnny Shines | 2:54 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |