| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Blue Yodel No. 2 (My Lovin' Gal Lucille) | Jimmie Rodgers | 3:17 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Texas Worried Blues | Henry Thomas | 3:18 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Alabama Jubilee | R. Crumb And His Cheap Suit Serenaders | 2:40 | |
| Bull Doze Blues | Henry Thomas | 3:32 | |
| Anniversary Blue Yodel (Blue Yodel No. 7) | Jimmie Rodgers | 2:53 | |
| Let Me Play with Your Poodle | Tampa Red | 2:39 | |
| Bye-Bye Blues | Tommy Johnson | 3:04 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| When the Sun Goes Down | Leroy Carr | 2:59 | |
| Long Haired Preachers (Preacher and the Slave) | Harry McClintock | 3:42 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
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| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| High Fever Blues | Bukka White | 2:50 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Cherry Ball Blues | Skip James | 2:52 | |
| Swing Low Sweet Chariot | Sister O.M. Terrell | 2:36 | |
| Blues Stay Away from Me | The Delmore Brothers | 2:49 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Busy Bootin' | Casey Bill Weldon, Kokomo Arnold | 2:27 | |
| Friar's Point Blues | Robert Nighthawk | 2:54 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| When the Levee Breaks - Alternate Take | Memphis Minnie | 3:09 | |
| Pin in Your Cushion | Bo Carter | 3:06 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Honey, Won't You Allow Me One More Chance? | Henry Thomas | 2:53 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| God Moves On the Water | Blind Willie Johnson | 2:59 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Lovin' Babe | Henry Thomas | 3:05 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Sagefield Woman Blues | Kokomo Arnold | 3:04 | |
| Catfish Blues | Skip James | 4:37 | |
| House Of The Risin' Sun - Rising Sun Blues | Clarence Ashley | 2:39 | |