| Levee Camp Blues | Son House | 3:45 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Mississippi Bottom Blues | Kid Bailey | 2:49 | |
| I've Got Blood In My Eyes For You | Mississippi Sheiks | 3:14 | |
| Roll and Tumble Blues - 1929 Version | Hambone Willie Newbern | 3:04 | |
| Misty Mornin' | Arthur Whetsol, Duke Ellington, Lonnie Johnson, Duke Ellington Cotton Club Orchestra | 3:19 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Black Train Blues | Bukka White | 2:56 | |
| Keep Drinkin' | Little Brother Montgomery | 3:04 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Catfish Blues (Alternate Take) | Robert Petway | 2:54 | |
| Freight Train to Ride | Louisiana Red | 3:28 | |
| T.B. Blues | Champion Jack Dupree | 3:42 | |
| Bukka Jitterburg Swing | Bukka White | 2:35 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| Things Have Changed | Big Maceo | 3:00 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Truckin' My Blues Away | Blind Boy Fuller | 3:08 | |
| My Black Mama, Pt. 1 | Son House | 3:05 | |
| How Deep is the Ocean | Lonnie Johnson | 2:19 | |
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| How Long | Frank Stokes | 3:18 | |
| Let Me Play With Your Noodle - Remastered | Tampa Red | 2:36 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Two Bugs and a Roach | Earl Hooker | 4:19 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| Little Boy Blues | Robert Lockwood, Jr. | 3:06 | |
| Kassie Jones, Pt. 1 | Furry Lewis | 3:07 | |
| Ash Tray Blues | Papa Charlie Jackson | 2:58 | |
| Death Letter Blues | Son House | 4:19 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| The Twelves (The Dirty Dozens) | Casey Bill Weldon, Kokomo Arnold | 3:11 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Anna Lou Blues | Tampa Red | 2:54 | |
| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Woke Up With the Blues In My Fingers | Lonnie Johnson | 3:11 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| Sagefield Woman Blues | Kokomo Arnold | 3:04 | |
| Story Of My Life | Guitar Slim | 3:00 | |
| Long Way Home | Clarence "Gatemouth" Brown | 5:38 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| Goin' Down Swingin' | Homesick James | 3:46 | |
| One For The Highway | James Booker | 2:34 | |
