| Lazy John | Chance McCoy & The Appalachian String Band | 2:59 | |
| East Tennessee Blues | Bill Monroe and Doc Watson | 2:12 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Single Girl | The Kossoy Sisters | 2:16 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Trouble on My Mind (1888 Luscomb Banjo) | Clifton Hicks | 2:27 | |
| John Hardy | Frank Proffitt | 2:27 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| How Can a Poor Man Stand Such Times and Live | The New Lost City Ramblers | 3:35 | |
| Captain Kidd | Michael Hurley | 2:16 | |
| She Left Me Standing On the Mountain | Tatiana Hargreaves, Sarah Jarosz, Alex Hargreaves, Bruce Molsky | 2:52 | |
| Drunkard's Lone Child | Clifton Hicks | 3:53 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Cumberland Gap | Frank Fairfield | 3:33 | |
| Dance All Night With A Bottle In Your Hand | Chance McCoy & The Appalachian String Band | 2:56 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| The Golden Vanity | Jody Stecher | 5:28 | |
| Rye Whiskey | Clifton Hicks | 4:25 | |
| Trouble in Your Mind | Carolina Chocolate Drops | 2:55 | |
| Cotton Eyed Joe | The New Lost City Ramblers | 1:15 | |
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| Black Eye Susie | Roscoe Holcomb | 1:14 | |
| Lazy John / The Bucking Mule | Bruce Molsky | 4:12 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| The Eighth of January - Remastered | Bookmiller Shannon, Alan Lomax | 0:56 | |
| Nobody's Business | Riley Puckett | 2:40 | |
| Took My Gal a-Walkin' | Charlie Poole | 2:48 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| A Lazy Farmer Boy | Buster Carter & Preston Young | 3:01 | |
| Wouldn't That Be Nice | Blaze Foley | 1:30 | |
| June Apple | Foghorn Stringband | 3:58 | |
| Hard Times on Beaver Dam Road (1888 Luscomb Banjo) | Clifton Hicks | 3:39 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:46 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| John Henry | Willie Watson | 2:45 | |
| No One Knows my Name | Nate Leath, Sarah Jarosz | 3:22 | |
| Soldier's Joy | The Rice Brothers | 1:34 | |
| Booth Shot Lincoln | Uncle Earl | 2:09 | |
| Davy Davy | Bob Carlin | 1:28 | |
| Ninety Nine Years (And One Dark Day) | Hot Rize | 3:08 | |
| Beaumont Rag | The Kentucky Colonels | 1:57 | |
| Little Maggie | The Doc Watson Family | 2:39 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |
| Grey Cat On The Tennessee Farm | Highwoods String Band | 3:43 | |
| Things In Life | Don Stover | 3:07 | |
| Sugarfoot Rag | Bill Keith | 1:50 | |