| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Utah Carl | Harry McClintock | 1:34 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Little Sadie | Hedy West, Bill Clifton | 2:55 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Drunkard's Special | Coley Jones | 3:15 | |
| The Wife Wrapt in Wether's Skin | Hedy West | 2:32 | |
| Swing and Turn Jubilee | Jean Ritchie and Doc Watson | 2:12 | |
| Mississippi County Farm Blues | Son House | 2:50 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Hello Stranger | Any Old Time String Band | 2:18 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| I Will Not Go Down Under the Ground (Let Me Die in My Footsteps) | Happy Traum with Bob Dylan | 3:41 | |
| If I Could Be the Rain | Rosalie Sorrels | 2:55 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
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| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Reuben Train | Frank Proffitt | 2:57 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| The Butcher Boy | Peggy Seeger | 2:20 | |
| Ira Hayes (Ballad of Ira Hayes) | Peter La Farge | 3:35 | |
| Banks of the Ohio | Bascom Lamar Lunsford | 2:48 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Dying Crapshooters Blues | Blind Willie McTell | 3:06 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Who's Gonna' Shoe Your Pretty Little Foot | Barbara Dane | 3:43 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| The House Carpenter - 2020 Remaster | Clarence Ashley | 3:18 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Look Down That Lonesome Road | The Doc Watson Family | 2:05 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Old Mountain Dew | The Delmore Brothers | 2:34 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| On A Bright And Summer's Morning | Bascom Lamar Lunsford | 2:15 | |
| Half Shaved / High Atmosphere | Wade Ward | 2:11 | |
| Italy | Bascom Lamar Lunsford | 3:26 | |
| Someday Soon | Ian & Sylvia | 2:16 | |