| Fishing Blues | Henry Thomas | 2:42 | |
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | |
| Jesus Won't You Come by Here | Barbara Dane, Lightnin' Hopkins | 2:07 | |
| Fixin' to Die Blues | Bukka White | 2:51 | |
| Breaking Up Ice in the Allegheny | Mike Seeger | 1:45 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| Turn Your Radio On (I) | John Hartford | 1:22 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Rye Whiskey | Frank Proffitt | 2:31 | |
| Long Tall Girl Got Stuck on Me | Mance Lipscomb | 2:43 | |
| Swing and Turn Jubilee | Jean Ritchie and Doc Watson | 2:12 | |
| John Brown | Hobart Smith | 1:51 | |
| Jesus Is My Friend | Washington Phillips | 2:53 | |
| Going Up On The Mountain | Jody Stecher | 2:45 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Peachbottom Creek | Wade Ward | 1:28 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Linin' Track | Spider John Koerner, Dave Ray, Tony Glover | 2:19 | |
| My Long Journey Home | The Monroe Brothers | 2:29 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
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| Single Girl | The Kossoy Sisters | 2:16 | |
| Sourwood Mountain | Wade Ward | 1:19 | |
| Nobody's Business | Riley Puckett | 2:40 | |
| Kidder Cole | Bascom Lamar Lunsford | 2:33 | |
| New River Train (Version 2) | Matokie Slaughter | 2:00 | |
| Beaver Dam Road | Frank Proffitt | 1:26 | |
| Pretty Polly | Dock Boggs | 2:56 | |
| Ruthie | Frank Fairfield | 3:39 | |
| When First Into this Country | The New Lost City Ramblers | 2:48 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Train A-Travelin' | Blind Boy Grunt (Bob Dylan) | 2:04 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Pretty Saro | Jean Ritchie and Doc Watson | 1:49 | |
| La Le Too Dum | Barbara Dane | 3:18 | |
| Buddy Won't You Roll Down the Line | Uncle Dave Macon | 3:12 | |
| The Fox and the Hounds | Henry Thomas | 2:36 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| The Lost Soul | The Doc Watson Family | 3:01 | |
| I Belong To The Band | Rev. Gary Davis | 2:54 | |
| Lulu Wall | Bascom Lamar Lunsford | 3:02 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Get Down On Your Knees And Pray | The Kentucky Colonels | 3:09 | |